This study concludes that there is a significant rise of Homocysteine level in patients of Acute Myocardial Infarction and this rise occurs not suddenly but over a long time when the disease is being in progress which ultimately reach to Acute Myocardial Infarction. The regular surveillance of plasma Homocysteine in patients above 40 years can be utilized for the possible prediction and prevention of Acute Myocardial Infarction.