This book investigated the biochemical parameters, activity levels of marker enzymes and serum electrolytes of Wistar albino rats fed with naturally ripe (control), unripe and artificially ripened mango pulp formulated diets. Ripe and unripe fruits were collected and used for the study and artificially ripened mangoes were obtained by wrapping unripe fruits with dark polyethylene bag; treatment with calcium carbide and by dipping into hot water. After ripening, the mango samples were air-dried, pulverized and used to formulate the 10%, 20%, 30% diets fed to rats for 28 days and their plasma collected for biochemical investigations. At 10% level of incorporation, plasma protein concentration in the control (65.63±0.12mg/dl) was significantly higher (p< 0.05) than values for unripe groups (55.50±1.91mg/dl) and calcium carbide (56.97±1.27mg/dl) while Albumin concentration was highest in the unripe (41.33±3.58mg/dl). Findings revealed that artificial ripening of fruits caused various alterations in Biochemical parameters, activity levels of marker enzymes and serum electrolytes especially for animals that were fed calcium carbide ripened mango formulated diets.