Biochemical Pathways and Environmental Responses in Plants, Part A, Volume 676 in the Methods in Enzymology series highlights new advances in the field with this new volume presenting interesting chapters on topics such as Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor…mehr
Biochemical Pathways and Environmental Responses in Plants, Part A, Volume 676 in the Methods in Enzymology series highlights new advances in the field with this new volume presenting interesting chapters on topics such as Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, and much more.
Other chapters cover Biochemical characterization of plant aromatic aminotransferases, Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more.
Prof. Jez received his B.S. in biochemistry from Penn State University (1992), a Ph.D. in biochemistry & molecular biophysics from the University of Pennsylvania (1998), and was an NIH-NRSA postdoctoral fellow at the Salk Institute for Biological Studies (1998-2001). After working as a research scientist at Kosan Biosciences (2001-2002), he started his research group at the Donald Danforth Plant Science Center and moved to the Department of Biology at Washington University in 2008. He has authored more than 160 papers and received a Presidential Early Career Award for Scientists and Engineers (2005), the Phytochemical Society of North America's Arthur Neish Young Investigator Award (2007), and a Fulbright Senior Specialist Award (2012) and was named as Howard Hughes Medical Institute Professor in 2014. Research in the Jez lab seeks to understand how environmental changes re-model biochemical pathways in plants at the molecular, cellular, and organism levels with the aim of engi
neering these systems to address agricultural and environmental problems. Current work in the lab employs a combination of structural biology, protein chemistry, and plant biology. He also launched the Biotech Explorers Program, which aims to introduce undergraduates to how teams in science can tackle real world problems using cross-disciplinary approaches.
Inhaltsangabe
1. Structure, function, and engineering of plant polyketide synthases Ikuro Abe 2. A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation N Bisht 3. Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay Sanja Roje 4. An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry Haijun Liu 5. Biochemical characterization of plant aromatic aminotransferases Hiroshi Maeda 6. Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites Soon Goo Lee 7. A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions Cynthia Holland 8. Plant metacaspase: an example of microcrystal structure determination and analysis Qun Liu 9. Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners Chang-Jun Liu 10. Dirigent Protein Family Function and Structure Norman G. Lewis 11. Lignin/Lignan Biosynthesis Structure/Function Norman G. Lewis 12. Discovery and characterization of BAHD acylsugar acyltransferases Robert L. Last 13. Near-real time determination of BAHD acyl-coenzyme A transferase reaction rates and kinetic parameters using Ellman's reagent Michael Sullivan 14. Quantification of N-terminal protein acetyltransferase activity in vitro and in planta Markus Wirtz 15. Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo Jeffrey Cameron 16. Analysis of Plant Alkaloid-Protein Interactions Using Differential Scanning Fluorimetry and Isothermal Titration Calorimetry Peter Facchini 17. A metabolomics strategy for plant specialized metabolites using liquid chromatography-mass spectrometry Kazuki Saito and Tetsuya Mori 18. Isoprene measurements to assess the methylerythritol pathway Thomas D. Sharkey 19. Analysis of isoprenoid alcohol phosphates by liquid chromatorgraphy-mass spectrometry Peter Dörmann and Katharina Gutbrod 20. Using targeted metabolomics to elucidate the indole auxin network in plants Jerry D. Cohen and Adrian D. Hegeman 21. Metabolite fingerprinting: A powerful metabolomics approach for marker identification and functional gene annotation Ivo Feussner 22. Ex vivo metabolomics, an hypothesis-free approach to identify native substrate(s) of orphan enzymes Kirstin Feussner 23. Using stable isotopes and LC-MS for precursor of origin determination in plants Clint Chapple 24. Measurement of flux through sulfate assimilation using [35S]sulfate Stanislav Kopriva 25. Assessment of the activity of sulfate uptake and assimilation in plants Rüdiger Hell 26. Comparison of TLC, HPLC, and direct infusion mass spectrometry methods for the identification and quantification of diacylglycerol molecular species Philip Bates 27. Plant sphingolipid analysis Jennifer E. Markham 28. Strategies to study the metabolic origins of plant specialized metabolites: the 1,4-naphthoquinones in Lithospermum erythrorhizon Joshua R. Widhalm 29. Controlled environments for highly reproducible cannabis 'omics' studies Bernd Markus Lange 30. Multi-platform approaches for the comprehensive analysis of aroma volatiles in cannabis Bernd Markus Lange 31. Systems and strategies for plant protein expression Thomas D. Niehaus 32. Programmable Chemical Actuator Control of Soluble and Membrane-Bound Enzymatic
1. Structure, function, and engineering of plant polyketide synthases Ikuro Abe 2. A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation N Bisht 3. Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay Sanja Roje 4. An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry Haijun Liu 5. Biochemical characterization of plant aromatic aminotransferases Hiroshi Maeda 6. Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites Soon Goo Lee 7. A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions Cynthia Holland 8. Plant metacaspase: an example of microcrystal structure determination and analysis Qun Liu 9. Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners Chang-Jun Liu 10. Dirigent Protein Family Function and Structure Norman G. Lewis 11. Lignin/Lignan Biosynthesis Structure/Function Norman G. Lewis 12. Discovery and characterization of BAHD acylsugar acyltransferases Robert L. Last 13. Near-real time determination of BAHD acyl-coenzyme A transferase reaction rates and kinetic parameters using Ellman's reagent Michael Sullivan 14. Quantification of N-terminal protein acetyltransferase activity in vitro and in planta Markus Wirtz 15. Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo Jeffrey Cameron 16. Analysis of Plant Alkaloid-Protein Interactions Using Differential Scanning Fluorimetry and Isothermal Titration Calorimetry Peter Facchini 17. A metabolomics strategy for plant specialized metabolites using liquid chromatography-mass spectrometry Kazuki Saito and Tetsuya Mori 18. Isoprene measurements to assess the methylerythritol pathway Thomas D. Sharkey 19. Analysis of isoprenoid alcohol phosphates by liquid chromatorgraphy-mass spectrometry Peter Dörmann and Katharina Gutbrod 20. Using targeted metabolomics to elucidate the indole auxin network in plants Jerry D. Cohen and Adrian D. Hegeman 21. Metabolite fingerprinting: A powerful metabolomics approach for marker identification and functional gene annotation Ivo Feussner 22. Ex vivo metabolomics, an hypothesis-free approach to identify native substrate(s) of orphan enzymes Kirstin Feussner 23. Using stable isotopes and LC-MS for precursor of origin determination in plants Clint Chapple 24. Measurement of flux through sulfate assimilation using [35S]sulfate Stanislav Kopriva 25. Assessment of the activity of sulfate uptake and assimilation in plants Rüdiger Hell 26. Comparison of TLC, HPLC, and direct infusion mass spectrometry methods for the identification and quantification of diacylglycerol molecular species Philip Bates 27. Plant sphingolipid analysis Jennifer E. Markham 28. Strategies to study the metabolic origins of plant specialized metabolites: the 1,4-naphthoquinones in Lithospermum erythrorhizon Joshua R. Widhalm 29. Controlled environments for highly reproducible cannabis 'omics' studies Bernd Markus Lange 30. Multi-platform approaches for the comprehensive analysis of aroma volatiles in cannabis Bernd Markus Lange 31. Systems and strategies for plant protein expression Thomas D. Niehaus 32. Programmable Chemical Actuator Control of Soluble and Membrane-Bound Enzymatic
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