This book is intended for students preparing a Biochemistry License, Fundamental Biochemistry License. It covers the essential aspects of biochemistry and metabolic regulation which we have classified into four chapters bringing together general concepts on the endocrine glands, carbohydrate metabolism, lipid metabolism and protein metabolism. We followed each chapter by the mode of regulation and metabolic diseases. In order to make it easier for students to assimilate basic knowledge in metabolic biochemistry, we have illustrated the different chapters with explanatory diagrams.