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In this Books, we focused on the isolation of indigenous fungi to degrade dye which is present as a pollutant in water.In this studied an attempt was made to isolate efficient bio-degradable fungi from dye contaminated soil and water sample. We isolate Cladosporium sp. and Aspergillus sp. fungi which ware employed for biodegradation of commercially used textile dyes such as Methyl Red (MR), Congo Red (CR) and Acid Black 210 (AB). The biodegradation of all dyes by fungi was studied in in minimal medium with fix concentration. Samples after decolorization were analyzed by Thin Layer Chromatography (TLC), Fourier Transform-Infrared spectroscopy (FT-IR), Mass Spectroscope (MS) Analysis.