The present research work is confined to identify botanical resources that can be used as biodiesel in Pakistan and to check their viability from technical, commercial and economic point of view. The study was conducted in Biodiesel Lab Department of Plant Sciences, Quaid-i-Azam University Islamabad. Research work was comprehensive and confined to identify the global and national resources for biodiesel production. The study mainly focused on detailed description of plant species, morphology, Anatomy, palynology, ecology, cultivation, harvesting and energy. Frequent field trips and surveys were conducted to collect the seeds of plant species and identify resource based areas. Data on production of seeds, oil yield, biodiesel production and production of byproducts of biodiesel were find out and presented in tables and photographic profiles. All these finding were systematically arranged and described in comprehensive descriptions, figures, tables and photographic plates. It is concluded that Pongamia pinnata is the highest source of Biodiesel than Ricinus communis and Sesamum indicum. However all the three plant species need cultivation on their suitable habitat in various parts