Contents: 1. Growing world energy demand 2. Biofuel production by the world 3. Sampling sites and sample collection4. Collection of raw materials 5. Enumeration of the actinobacteria 6. Cultural characteristic of actinobacteria strain 7. Oxidative fermentation test 8. Carbohydrate fermentation test at anaerobic incubation, Acid production and Acid + gas production 9 Quantitative analysis of biogas using different substrates 10. GC analysis of sugarcane molasses fermentation 11. GC analysis of cassava waste fermentation 12. GC analysis of food waste fermentation 13. Biogas purification system 14. Before gas collection, after gas collection 15. GC analysis of purified hydrogen biogas. 16. HPLC analysis of hydrogen-fermented medium 17. Effect of various nitrogen sources for the biohydrogen production using SM118. Effect of various mineral sources for the biohydrogen production using SM1 19. Effect of different sugarcane molasses concentrations on the hydrogen production 20. Effect of different peptone concentrations on the hydrogen production 21. Effect of different MgSO4 concentrations on the hydrogen production22. Effect of different pH on the hydrogen production using actinobacteria SM1 23. Effect of different temperatures on hydrogen production using actinobacteria SM124. Effect of different inoculum concentrations on hydrogen production using actinobacteria SM1 25. Michaelis-Menten (M-M) plot on biohydrogen SM1 for different concentrations of sugarcane molasses26. Michaelis-Menten (M-M) plot on biohydrogen SM1 for different pH 27. Michaelis-Menten (M-M) plot on biohydrogen SM1 for different temperature 28. Mass production of biohydrogen by dark fermentation method using actinobacteria SM1 29. Primary screening of bioethanol production by actinobacteria 30. Standard glucose estimation graph 31. Standard ethanol estimation graph 32. Effect of different nitrogen sources on bioethanol production using actinobacteria SM18 33. Effect of different mineral sources on bioethanol production using actinobacteria SM18 34. Effect of different substrate concentrations on bioethanol production using actinobacteria SM18 35. Effect of different yeast extract concentrations on the bioethanol production using actinobacteria SM18 36. Effect of different MgSO4 concentrations on the bioethanol production using actinobacteria SM18 37. Effect of different pH on the bioethanol production using actinobacteria SM18 38. Effect of different temperatures on bioethanol production using actinobacteria SM18 39. Effect of different inoculum sizes on the bioethanol production using actinobacteria SM18 40. Effect of different incubation times on the bioethanol production using actinobacteria SM18 41. Three-dimensional (3D) response surface plots for ethanol production with different factors A, B, C, and D 42. Three-dimensional (3D) response surface plots for saccharification with different factors A, B, C, and D 43. Mass production of bioethanol by potential strain SM18 44. Distillation of bioethanol by potential strain SM18 45. Characterization of bioethanol using FTIR analysis 46. Gas chromatography analysis of standard ethanol 47. Gas chromatography analysis of distilled bioethanol 48. GCMS-Chromatogram of distilled bioethanol 49. GCMS-Mass spectrum of bioethanol 50. Structure of ethanol content 51. Starch hydrolysis by actinobacteria 52. Standard glucose estimation graph 53. Effect of different nitrogen sources for reducing sugar production and saccharification using actinobacteria SM1 54 Effect of different mineral sources for reducing sugar production and saccharification using actinobacteria SM1
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