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This book consists of preparation,usage and effect of bioinoculants including phosphate solubilizing bacteria (PSB), Nitrogen fixing bacteria (Azatobacter sp.)and VAM fungi (Glomus sp.)on growth and biochemical aspects of Stevia rebaudiana (Bert.) plant- an emerging nutraceutical plant. Elaborate description and protocols of growth and various biochemical parameters were given with detailed explanations.Particularly carbohydrates and bioactive compounds i.e. "Steviosides" isolation and estimation were well explained. It includes the soil analysis and VAM colonization with the studied plant. Micro nutrients and macro nutrients analysis in both plant and rhizosphere soil were studied and provided with results. This work was the first of its kind that reports VAM colonization in Stevia plant. It also contains important enzymes activity studies and their variations with the applied bioinoculants. The book will be a reference for researchers in the field of bioinoculants studies and their effect on nutraceutical plants.