This in-depth book on Clostridium botulinum and its neurotoxin, botulinum toxin, explores in detail the biological and chemical aspects of this anaerobic bacterium. Focusing on bioinorganic chemistry, it examines how Clostridium botulinum uses metals and metalloenzymes to metabolize and protect itself in hostile environments. The structure and mechanism of action of botulinum toxin are explained in detail, revealing how it interferes with nerve transmission for innovative applications. Ethical considerations and regulatory challenges are addressed, as well as recent technological advances that enhance its efficacy and safety. In conclusion, this book offers an in-depth, interdisciplinary exploration of Clostridium botulinum and botulinum toxin, highlighting their continuing potential for scientific research and technological innovation.