Bioluminescence

Methods and Protocols
Herausgegeben:Kim, Sung Bae

• Gebundenes Buch

Produktbeschreibung

This volumepresents detailed laboratory protocols regarding the three major routetechnologies luciferases, efficient opticalprobes, and applications to visualizing molecular events in living subjects. Bioluminescence: Methods and Protocols, Third Edition guides readers through chapters on ingredients of bioluminescent probes, and fabrication of bioluminescent probes, applications to living subjects and instrumentations. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Bioluminescence: Methods and Protocols, Third Edition is a usefulcomplement to the first and second edition for new and experienced researchersalike.

Produktdetails

• Methods in Molecular Biology 1461
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-3811-7
• 3. Aufl.
• Seitenzahl: 328
• Erscheinungstermin: 18. Juli 2016
• Englisch
• Abmessung: 260mm x 183mm x 24mm
• Gewicht: 745g
• ISBN-13: 9781493938117
• ISBN-10: 1493938118
• Artikelnr.: 44711200

Inhaltsangabe

Label-free Cell Phenotypic Identificationof D-Luciferin as an Agonist for GPR35.- Synthetic BioluminescentCoelenterazine Derivatives.- Molecular Cloning of SecretedLuciferases from Marine Planktonic Copepods.- How to Fabricate FunctionalArtificial Luciferases for Bioassays.- QuantumYield Determination Based on Photon Number Measurement, Protocols for FireflyBioluminescence Reactions.- Bioluminescent Ligand-ReceptorBinding Assays for Protein or Peptide Hormones.- Bioluminogenic Imaging of AminopeptidaseN in vitro and in vivo.- Firefly Luciferase-basedSequential Bioluminescence Resonance Energy Transfer (BRET)-FluorescenceResonance Energy Transfer (FRET) Protease Assays.- Monitoring Intracellular pH Changewith a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast andMammalian Cells.- A Protein-Protein InteractionAssay FlimPIA Based on the Functional Complementation of Mutant FireflyLuciferases.- Single-chain Probes for IlluminatingAndrogenicity of Chemicals.- MulticolorImaging of Bifacial Activities of Estrogens.- CircularPermutation Probes for Illuminating Phosphorylation of Estrogen Receptor.- Fabricationof Molecular Strain Probes for Illuminating Protein-Protein Interactions.- AnALuc-based Molecular Tension Probe for Sensing Intramolecular Protein-Protein Interactions.- Live Cell Bioluminescence Imagingin Temporal Reaction of G Protein-coupled Receptor for High-throughput Screeningand Analysis.- Imaging Histone Methylations inLiving Animals.- Preparation and Assay of Simple Light off Biosensor Based on ImmobilizedBioluminescent Bacteria for General Toxicity Assays.- InVivo Bioluminescent Imaging of ATP-BindingCassette Transporter-Mediated Efflux at the Blood-Brain Barrier.- Theranostic Imaging of CancerGene Therapy.- Development of a Multicolor BioluminescenceImaging Platform to Simultaneously Investigate Transcription Factor NF- B Signalingand Apoptosis .- A Multichannel BioluminescenceDetermination platform for Bioassays.- A Bioluminescence Assay Systemfor Imaging Metal Cationic Activities in Urban Aerosols.- LuminescenceImaging: (a) Multi-color Visualization of Ca²⁺ Dynamics in DifferentCellular Compartments and (b) Video-rate Tumour Detection in a Freely Moving Mouse.- Photon Counting System for High-sensitivityDetection of Bioluminescence at Optical Fiber End.