Biophysical Tools for Biologists
In Vitro Techniques Volume 84
Herausgegeben:Correia, John J.; Detrich, III, H. William
Biophysical Tools for Biologists
In Vitro Techniques Volume 84
Herausgegeben:Correia, John J.; Detrich, III, H. William
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Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vitro and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell…mehr
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Describes techniques in the context of important biological problems Delineates critical steps and potential pitfalls for each method Includes full-color plates to illustrate techniques
- Produktdetails
- Methods in Cell Biology 84
- Verlag: Academic Press
- Artikelnr. des Verlages: 19681311
- Seitenzahl: 1014
- Erscheinungstermin: 1. Oktober 2007
- Englisch
- Abmessung: 235mm x 199mm x 39mm
- Gewicht: 1842g
- ISBN-13: 9780123725202
- ISBN-10: 0123725208
- Artikelnr.: 23081374
- Methods in Cell Biology 84
- Verlag: Academic Press
- Artikelnr. des Verlages: 19681311
- Seitenzahl: 1014
- Erscheinungstermin: 1. Oktober 2007
- Englisch
- Abmessung: 235mm x 199mm x 39mm
- Gewicht: 1842g
- ISBN-13: 9780123725202
- ISBN-10: 0123725208
- Artikelnr.: 23081374
Section 1. Solution Methods
1. Binding: A Polemic and Rough Guide
Nichola C. Garbett, Jonathan B. Chaires
2. Linked Equilibria in Regulation of Transcription Initiation
Dorothy Beckett
3. Biosensor-Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular Interactions
Farial A. Tanious, Binh Nguyen, and W. David Wilson
4. Isothermal Titration Calorimetry: Experimental Design, Data Analysis, and Probing Macromolecule/Ligand Binding and Kinetic Interactions
Matthew W. Freyer and Edwin A. Lewis
5. Differential Scanning Calorimetry
Charles H. Spink
6. Analytical Ultracentrifugation: A) Sedimentation Velocity, B) Sedimentation Equilibrium
Tom Laue, Tom Moody, James Cole, and Jeff Lary
7. Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity
Nancy K. Burgess, Ann Marie Stanley, and Karen G. Fleming
8. Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer Methods
Susan Bane and Natasha Shanker
9. Applications of Fluorescence Anisotropy to the Study of Protein-DNA Interactions
Vince J. LiCata and Andy J. Wowor
10. Circular Dichroism and Its Application to the Study of Biomolecules
Stephen R. Martin and Maria J. Schilstra
11. Folding and Stability
Timothy O. Street, Naomi Courtemanche and Doug Barrick
12. Hydrodynamic shape modelling of analytical ultracentrifugation data
Olwyn Byron
13. X-ray and neutron scattering data and their constrained molecular modelling
Stephen J Perkins, Azubuike I. Okemefuna, Anira N. Fernando, Alexandra Bonner, Hannah E. Gilbert and Patricia B. Furtado
14. Structural Investigations into Microtubule-MAP Complexes
Andreas Hoenger and Heinz Gross
15. Rapid Kinetic Techniques
John F. Eccleston, Stephen Martin, and Maria J. Schilstra
16. Mutagenic Analysis of the Membrane Protein Functional Mechanisms: Bacteriorhodopsin as a Model Example
George J. Turner
17. Quantifying DNA-protein interactions by single molecule stretching
Mark C. Williams, Ioulia Rouzina, and Richard L. Karpel
18. Isotopomer-based metabolomic analysis by NMR and mass spectrometry
Andrew N Lane, Teresa W-M. Fan, and Richard M. Higashi
19. Following molecular transitions with single residue spatial
and millisecond time resolution
Inna Shcherbakova, Somdeb Mitra, Robert Beer and Michael Brenowitz
20. Methods and applications of site-directed spin labeling EPR spectroscopy
Candice S. Klug and Jimmy B. Feix
21. Fluorescence Correlation Spectroscopy and its application to the characterization of molecular properties and interactions
Hacène Boukari and Dan L. Sackett
22. Practical Guide to Osmolytes
Jorg Rosgen and Daniel Harries
23. Stupid Statistics
Joel Tellinghuisen
24. Nonlinear Least Squares Fitting Methods
Michael L. Johnson
25. Methods for Simulating the Dynamics of Complex Biological Processes
Maria J Schilstra, Stephen R. Martin, and Sarah M. Keating
26. Computational Methods for Biomolecular Electrostatics
Feng Dong, Brett Olsen and Nathan A. Baker
27. Ligand effects on the protein ensemble: Unifying the descriptions of ligand binding, local conformational fluctuations, and protein stability
Steven T Whitten, Bertrand García-Moreno E., and Vincent J. Hilser
28. Molecular Modeling of Cytoskeletal Proteins
Xiange Zheng and David Sept
29. Mathematical Modeling of Cell Motility
Anders E. Carlsson and David Sept
Section 1. Solution Methods
1. Binding: A Polemic and Rough Guide
Nichola C. Garbett, Jonathan B. Chaires
2. Linked Equilibria in Regulation of Transcription Initiation
Dorothy Beckett
3. Biosensor-Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular Interactions
Farial A. Tanious, Binh Nguyen, and W. David Wilson
4. Isothermal Titration Calorimetry: Experimental Design, Data Analysis, and Probing Macromolecule/Ligand Binding and Kinetic Interactions
Matthew W. Freyer and Edwin A. Lewis
5. Differential Scanning Calorimetry
Charles H. Spink
6. Analytical Ultracentrifugation: A) Sedimentation Velocity, B) Sedimentation Equilibrium
Tom Laue, Tom Moody, James Cole, and Jeff Lary
7. Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity
Nancy K. Burgess, Ann Marie Stanley, and Karen G. Fleming
8. Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer Methods
Susan Bane and Natasha Shanker
9. Applications of Fluorescence Anisotropy to the Study of Protein-DNA Interactions
Vince J. LiCata and Andy J. Wowor
10. Circular Dichroism and Its Application to the Study of Biomolecules
Stephen R. Martin and Maria J. Schilstra
11. Folding and Stability
Timothy O. Street, Naomi Courtemanche and Doug Barrick
12. Hydrodynamic shape modelling of analytical ultracentrifugation data
Olwyn Byron
13. X-ray and neutron scattering data and their constrained molecular modelling
Stephen J Perkins, Azubuike I. Okemefuna, Anira N. Fernando, Alexandra Bonner, Hannah E. Gilbert and Patricia B. Furtado
14. Structural Investigations into Microtubule-MAP Complexes
Andreas Hoenger and Heinz Gross
15. Rapid Kinetic Techniques
John F. Eccleston, Stephen Martin, and Maria J. Schilstra
16. Mutagenic Analysis of the Membrane Protein Functional Mechanisms: Bacteriorhodopsin as a Model Example
George J. Turner
17. Quantifying DNA-protein interactions by single molecule stretching
Mark C. Williams, Ioulia Rouzina, and Richard L. Karpel
18. Isotopomer-based metabolomic analysis by NMR and mass spectrometry
Andrew N Lane, Teresa W-M. Fan, and Richard M. Higashi
19. Following molecular transitions with single residue spatial
and millisecond time resolution
Inna Shcherbakova, Somdeb Mitra, Robert Beer and Michael Brenowitz
20. Methods and applications of site-directed spin labeling EPR spectroscopy
Candice S. Klug and Jimmy B. Feix
21. Fluorescence Correlation Spectroscopy and its application to the characterization of molecular properties and interactions
Hacène Boukari and Dan L. Sackett
22. Practical Guide to Osmolytes
Jorg Rosgen and Daniel Harries
23. Stupid Statistics
Joel Tellinghuisen
24. Nonlinear Least Squares Fitting Methods
Michael L. Johnson
25. Methods for Simulating the Dynamics of Complex Biological Processes
Maria J Schilstra, Stephen R. Martin, and Sarah M. Keating
26. Computational Methods for Biomolecular Electrostatics
Feng Dong, Brett Olsen and Nathan A. Baker
27. Ligand effects on the protein ensemble: Unifying the descriptions of ligand binding, local conformational fluctuations, and protein stability
Steven T Whitten, Bertrand García-Moreno E., and Vincent J. Hilser
28. Molecular Modeling of Cytoskeletal Proteins
Xiange Zheng and David Sept
29. Mathematical Modeling of Cell Motility
Anders E. Carlsson and David Sept