W. Zhou

Bioprocessing Technology for Production of Biopharmaceuticals and Bioproducts

Herausgeber: Komives, Claire; Zhou, Weichang

• Gebundenes Buch

Produktbeschreibung

In the wake of rapid advances in genetic technologies, new products continue to be developed to help improve human health and quality of life. Summarizing state-of-the art bioprocessing methods, Novel Bioprocessing Technology for Production of Biopharmaceuticals and Bioproducts presents a concise exploration of the latest developments in bioprocessing for applications in both the biopharmaceutical and biochemical industries. Including case study reviews of six milestone byproducts, the authors provide industrial and academic researchers and development scientists and students with a wide selection of host strain types and a review disruptive bioprocess technologies.

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Produktdetails

• Wiley Series on Biotechnology
• Verlag: Wiley & Sons
• 1. Auflage
• Seitenzahl: 288
• Erscheinungstermin: 27. Dezember 2018
• Englisch
• Abmessung: 231mm x 155mm x 18mm
• Gewicht: 590g
• ISBN-13: 9781118361986
• ISBN-10: 1118361989
• Artikelnr.: 37320813

Autorenporträt

CLAIRE KOMIVES, PHD, is a Professor in the Chemical and Materials Engineering Department at San Jose State University. Her research interests focus on the development of low cost snake antivenom compounds. WEICHANG ZHOU, PHD, is Chief Technology Officer, Biologics Development & Manufacturing at WuXi Biologics. He has previously worked at Genzyme Corporation and Merck Research Laboratories.

Inhaltsangabe

List of Contributors xi Part I Case Study 1 1 Bacillus and the Story of
Protein Secretion and Production 3 Giulia Barbieri, Anthony Calabria, Gopal
Chotani, and Eugenio Ferrari 1.1 Bacillus as a Production Host:
Introduction and Historical Account 3 1.2 The Building of a Production
Strain: Genetic Tools for B. subtilis Manipulation 5 1.2.1 Promoters 5
1.2.2 Vectors for Building a Production Strain 6 1.2.3 B. subtilis
Competent Cell Transformation 7 1.2.4 Protoplasts-Mediated Manipulations 9
1.2.5 Genetics by Electroporation 9 1.3 B. subtilis Secretion Systemand
Heterologous Protein Production 9 1.3.1 Bacillus Fermentation and Recovery
of Industrial Enzyme 11 1.3.2 Fermentation Stoichiometry 12 1.3.3 Fermentor
Kinetics and Outputs 14 1.3.4 Downstream Processing 17 1.4 Summary 21
References 21 2 New Expression Systems for GPCRs 29 Dimitra Gialama,
Fragiskos N. Kolisis, and Georgios Skretas 2.1 Introduction 29 2.2
Recombinant GPCR Production - Traditional Approaches for Achieving
High-Level Production 39 2.3 Engineered Expression Systems for GPCR
Production 42 2.3.1 Bacteria 42 2.3.2 Yeasts 48 2.3.3 Insect Cells 51 2.3.4
Mammalian Cells 54 2.3.5 Transgenic Animals 54 2.3.6 Cell-Free Systems 56
2.4 Conclusion 57 References 58 3 Glycosylation 71 Maureen Spearman, Erika
Lattová, Hélène Perreault, andMichael Butler 3.1 Introduction 71 3.2 Types
of Glycosylation 72 3.2.1 N-linked Glycans 72 3.2.2 O-linked Glycans 74 3.3
Factors Affecting Glycosylation 76 3.3.1 Nutrient Depletion 76 3.3.2
Fed-batch Cultures and Supplements 79 3.3.3 Specific Culture Supplements 80
3.3.4 Ammonia 82 3.3.5 pH 82 3.3.6 Oxygen 83 3.3.7 Host Cell Systems 83
3.3.8 Other Factors 85 3.4 Modification of Glycosylation 86 3.4.1 siRNA and
Gene Knockout/Knockin 86 3.4.2 Glycoprotein Processing Inhibitors and In
Vitro Modification of Glycans 88 3.5 Glycosylation Analysis 89 3.5.1
Release of Glycans from Glycoproteins 90 3.5.2 Derivatization of Glycans 91
3.6 Methods of Analysis 91 3.6.1 Lectin Arrays 91 3.6.2 Liquid
Chromatography 93 3.6.2.1 HILIC Analysis 93 3.6.2.2 Reversed Phase (RP) and
Porous Graphitic Carbon (PGC) Chromatography 95 3.6.2.3 Weak Anion Exchange
(WAX) HPLC Analysis 96 3.6.2.4 High pH Anion Exchange Chromatography with
Pulsed Amperometric Detection (HPAEC-PAD) 96 3.6.3 Capillary
Electrophoresis (CE) 97 3.6.4 Fluorophore-assisted Carbohydrate
Electrophoresis (FACE) and CGE-LIF 99 3.6.5 Mass Spectrometry (MS) 100
3.6.5.1 Ionization 100 3.6.5.2 Derivatization Techniques Used for MS
Analysis of Glycans 102 3.6.5.3 Fragmentation of Carbohydrates 103 3.7
Conclusion 109 References 109 Part II Bioreactors 131 4 Bioreactors for
StemCell and Mammalian Cell Cultivation 133 Ana Fernandes-Platzgummer, Sara
M. Badenes, Cláudia L. da Silva, and JoaquimM. S. Cabral 4.1 Overview of
(Mammalian and Stem) Cell Culture Engineering 133 4.1.1 Cell Products for
Therapeutics 134 4.1.2 Cell as a Product: Stem Cells 136 4.2 Bioprocess
Characterization 140 4.2.1 Cell Cultivation Methods 140 4.2.2 Cell
Metabolism 141 4.2.3 Culture Medium Design 143 4.2.4 Culture Parameters 144
4.2.5 Culture Modes 145 4.3 Cell Culture Systems 147 4.3.1 Static Culture
Systems 147 4.3.2 Roller Bottles 150 4.3.3 Spinner Flask 150 4.3.4 Airlift
Bioreactor 151 4.3.5 Fixed/Fluidized-Bed Bioreactor 152 4.3.6 Wave
Bioreactor 152 4.3.7 Rotating-Wall Vessel Bioreactor 154 4.3.8 Stirred Tank
Bioreactor 155 4.3.8.1 Agitation/Shear Stress 156 4.4 Cell Culture Modeling
157 4.5 Case Studies 159 4.5.1 Antibody Production in Bioreactor Systems
159 4.5.2 mESC Expansion on Microcarriers in a Stirred Tank Bioreactor 161
4.6 Concluding Remarks 162 List of Symbols 163 References 164 5 Model-Based
Technologies Enabling Optimal Bioreactor Performance 175 Rimvydas Simutis,
Marco Jenzsch, and Andreas Lübbert 5.1 Introduction 175 5.2 Basics 176
5.2.1 Balances 176 5.2.2 Model Identification 177 5.2.3 Model-Based Process
Optimization 178 5.3 Examples 180 5.3.1 Model-Based State Estimation 180
5.3.1.1 Static Model Approach 180 5.3.1.2 Dynamic Alternatives 183 5.3.2
Optimizing Open Loop-Controlled Cultivations 184 5.3.2.1 Robust Cultivation
Profiles 184 5.3.2.2 Evolutionary Modeling Approach 188 5.3.3 Optimization
by Model-Aided Feedback Control 190 5.3.3.1 Improving the Basic Control 190
5.3.3.2 Optimizing the Amount of Soluble Product 190 5.3.4 CO2-Removal in
Large-Scale Cell Cultures 194 5.4 Conclusion 197 References 198 6
Monitoring and Control of Bioreactor: Basic Concepts and Recent Advances
201 James Gomes, Viki Chopda, and Anurag S. Rathore 6.1 Introduction 201
6.2 Challenges in Bioprocess Control 202 6.2.1 Process Dynamics and
Modeling 202 6.2.2 Limits of Hardware and Software and Their Integration
203 6.2.3 Regulatory Aspects 204 6.3 Basic Elements of Bioprocess Control
205 6.3.1 Bioprocess Monitoring 205 6.3.2 Parameter Estimators 205 6.3.3
Bioprocess Modeling 206 6.4 Current Practices in Bioprocess Control 208
6.4.1 PID Control 208 6.4.2 Model-Based Control 209 6.4.3 Adaptive Control
211 6.4.4 Nonlinear Control 214 6.5 Intelligent Control Systems 217 6.5.1
Fuzzy Control 217 6.5.2 Neural Control 219 6.5.3 Statistical Process
Control 222 6.5.4 Integrated and Plant-Wide Bioprocess Control 224 6.5.5
Metabolic Control 225 6.6 Summary 226 6.7 Future Perspectives 227
Acknowledgments 227 References 227 Part III Host Strain Technologies 239 7
Metabolic Engineering for Biocatalyst Robustness to Organic Inhibitors 241
Liam Royce and Laura R. Jarboe 7.1 Introduction 241 7.2 Mechanisms of
Inhibition 243 7.3 Mechanisms of Tolerance 245 7.4 Membrane Engineering 246
7.5 Evolutionary and Metagenomic Strategies for Increasing Tolerance 251
7.6 Reverse Engineering of Improved Strains 254 7.7 Concluding Remarks 255
Acknowledgments 255 References 255 Index 267