David W. Burden, Donald B. Whitney

Biotechnology Proteins to PCR

A Course in Strategies and Lab Techniques

• Broschiertes Buch

Produktbeschreibung

ism (i. e. , Saccharomyces carlsbergensis, or brewer's yeast) and one of its corresponding enzymes. The experiments on this organism and enzyme are not limited to the materials suggested and can be easily adapted to the desired technical level and available budget. Similarly, the subse quent cloning experiments suggest that use of particular vectors and strains, but, as indicated, alternative materials can be used to success fully perform the laboratory exercises. We would like to thank the corporate sponsors of the Biotechnology Training Institute for providing the materials and expertise for the devel opment of our programs, and thus for the materials in this manual. These sponsors include: Barnsteadffhermolyne, Dubuque, IA Beckman Instruments, Somerset, NJ Bio-Rad Laboratories, Hercules, CA Boehringer Mannheim Corporation, Indianapolis, IN Coming Costar Corporation, Cambridge, MA FMC BioProducts, Rockland, ME Kodak Laboratory Products, New Haven, CT Labconco, Kansas City, MO MJ Research, Cambridge, MA Olympus Instruments, Lake Success, NY Pharmacia Biotech, Piscataway, NJ Savant, Inc. , Farmingdale, NY VWR Scientific, Philadelphia, P A We would also like to thank the following individuals for their input, comments, and suggestions: Tom Slyker, Bernie Janoson, Steven Piccoli, John Ford,JeffGarelik, Yanan Tian, and Douglas Beecher. Special thanks to Alan Williams for his critique of the chromatography experiments and Shannon Gentile for her work in the laboratory. We would especial ly like to thank Maryann Burden for her comments and encouragement.

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Produktdetails

• Verlag: Birkhäuser / Birkhäuser Boston / Springer, Basel
• Artikelnr. des Verlages: 978-0-8176-3843-6
• 1995.
• Seitenzahl: 336
• Erscheinungstermin: 1. November 1995
• Englisch
• Abmessung: 254mm x 178mm x 19mm
• Gewicht: 646g
• ISBN-13: 9780817638436
• ISBN-10: 0817638431
• Artikelnr.: 22365167

Inhaltsangabe

1. Introduction to the Biotechnology Laboratory.- 1.1Overview.- 1.2 Background.- 1.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 2. Introduction to Proteins.- 2.1 Overview.- 2.2 Background.- 2.3 Experimental Design and Procedures.- Biuret Protein Assay.- Lowry Protein Assay.- Study Questions.- Further Readings.- 3. Protein Isolation and Preparation of Crude Extract.- 3.1 Overview.- 3.2 Background.- 3.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 4. Batch Purification of Proteins.- 4.1 Overview.- 4.2 Background.- 4.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 5. Protein Purification by Column Chromatography.- 5.1 Overview.- 5.2 Background.- 5.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 6. Protein Analysis and Verification.- 6.1 Overview.- 6.2 Background.- 6.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 7. Designing a Cloning Scheme.- 7.1 Overview.- 7.2 Background.- 73 Experimental Design and Procedures.- 8. Isolation and Preparation of Nucleic Acids.- 8.1 Overview.- 8.2 Background.- 8.3 Expermental Design and Procedures.- Study Questions.- Further Readings.- 9. Constructing a Gene Bank.- 9.1 Overview.- 9.2 Background.- 9.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 10. Introducing Recombinant Molecules into Escherichia coli.- 10.1 Overview.- 10.2 Background.- 10.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 11. Screening for Clones.- 11.1 Overview.- 11.2 Background.- 11.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 12. Characterizing and Verifying Cloned DNA.- 12.1 Overview.- 12.2 Background.- 12.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 13. Application of DNA Sequence and Clone Data.- 13.1 Overview.- 13.2 Background.- 13.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 14. The Value of Information.