A study was conducted on botanicals from various plant samples by extracting them into leaf powder to evaluate and identify their antifungal activity against Fusarium oxysporum, the causative agent of wilting in tomatoes. Four different plants were carefully chosen based on their potential for extraction to conduct the experiment. The efficacy of the plant samples varied due to their differing antifungal properties. Each plant sample demonstrated a different percentage of wilting in tomato plants, attributed to Fusarium oxysporum f.sp lycopersici.