Bovine mastitis is considered to be the main disease affecting dairy herds worldwide, causing serious economic damage to dairy cattle production worldwide. Among the most prevalent pathogens associated with mastitis in lactating cows are bacteria belonging to the genus Staphylococcus. The role of virulence factors of the Staphylococcus genus, such as coagulase and biofilm production, in the persistence and dissemination in the host, in the expression of severe disease and in the interference of antimicrobial treatment is currently being discussed.