Carbohydrate-Based Vaccines

Methods and Protocols
Herausgegeben:Lepenies, Bernd

• Broschiertes Buch

Produktbeschreibung

This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Carbohydrate-Based Vaccines: Methods and Protocols aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines.

Produktdetails

• Methods in Molecular Biology 1331
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-4919-9
• Softcover reprint of the original 1st ed. 2015
• Seitenzahl: 268
• Erscheinungstermin: 15. Oktober 2016
• Englisch
• Abmessung: 254mm x 178mm x 14mm
• Gewicht: 571g
• ISBN-13: 9781493949199
• ISBN-10: 1493949195
• Artikelnr.: 46432888

Inhaltsangabe

Carbohydrate-based Vaccines: An Overview.- Glycans as Vaccine Antigens and Adjuvants: Immunological Considerations.- The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens.- Antibody-carbohydrate Recognition from Docked Ensembles using the AutoMap Procedure.- Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens.- Murine Whole-Blood Opsonophagocytosis Assay to Evaluate Protection by Antibodies Raised Against Encapsulated Extracellular Bacteria.- Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility.- N-Glycosylation Fingerprinting of Viral Glycoproteins by xCGE-LIF.- Temporary Conversion of Protein Amino Groups To Azides: A Synthetic Strategy For Glycoconjugate Vaccines.- Gold Nanoparticles as Carriers For Synthetic Glycoconjugate Vaccines.- Identification and Characterization of Carbohydrate-Based Adjuvants.- Characterization of Carbohydrate Vaccines by NMR Spectroscopy Francesco Berti and Neil Ravenscroft.- Characterisation of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods.- Glycoconjugate Vaccines: The Regulatory Framework.