This book discusses systematic green synthesis of CuO Nanoparticles using leaf extract of Catha edulis. It also covers qualitative phytochemical screening of aqueous leaf extract of Catha edulis. More importantly it also explains characterization techniques of the as synthesized CuO Nanoparticles at minimum cost. Finally the synthesized CuO Nanoparticles was used for antibacterial application and also recommended for other applications.