In this work different studies including spectral and capillary electrophoretic methods have been conducted for characterization of a new class of boronic acid-based squarylium dyes for its utility as fluorescent probes for some biological and even biopharmaceutical targets. It describes the theoretical background and literature review of CE technique, instrumentation, modes, detection methods coupled with this technique specially LIF beside a brief introduction about derivatization and labeling protocols for analytes of interest.