Cell and Tissue Engineering of Cartilage - Khaghani, Seyed Ali (Behruz)
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Cartilage cell (Chondrocyte) is embedded in the matrix (Lacunae) and has round shape in vivo. The in vitro monolayer culture of primary chondrocyte causes morphological change characterized as dedifferentiation. Transforming growth factor-beta (TGF- ), a cytokine superfamily, regulates cell function, including differentiation and proliferation. The effect of TGF- 1, 2, 3, and their manipulated forms in biological regulation of primary chondrocyte was investigated in this work. A novel method was developed to isolate and purify the primary chondrocytes from knee joint of neonate Sprague-Dawley…mehr

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Produktbeschreibung
Cartilage cell (Chondrocyte) is embedded in the matrix (Lacunae) and has round shape in vivo. The in vitro monolayer culture of primary chondrocyte causes morphological change characterized as dedifferentiation. Transforming growth factor-beta (TGF- ), a cytokine superfamily, regulates cell function, including differentiation and proliferation. The effect of TGF- 1, 2, 3, and their manipulated forms in biological regulation of primary chondrocyte was investigated in this work. A novel method was developed to isolate and purify the primary chondrocytes from knee joint of neonate Sprague-Dawley rat, and the effect of some supplementations such as hyaluronic acid and antibiotics were also investigated to provide the most appropriate condition for in vitro culture of chondrocyte cells.
Autorenporträt
SA (Behruz) Khaghani, PhD (TE): Studied industry, mechanical and medical engineering in Turkey, Germany and United Kingdom respectively. He received PhD in cell and tissue engineering from University of Bradford and worked in industrial and large organisations for more than 18 years. He is currently a research fellow in cell and tissue engineering.