Cell-Based Assays for High-Throughput Screening

Methods and Protocols
Herausgegeben:Clemons, Paul A.; Tolliday, Nicola J.; Wagner, Bridget K.

• Gebundenes Buch

Produktbeschreibung

As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology(TM) series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.

Produktdetails

• Methods in Molecular Biology 486
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 12250929, 978-1-60327-544-6
• 2009
• Seitenzahl: 232
• Erscheinungstermin: 28. April 2009
• Englisch
• Abmessung: 266mm x 198mm x 18mm
• Gewicht: 702g
• ISBN-13: 9781603275446
• ISBN-10: 1603275444
• Artikelnr.: 24431446

Inhaltsangabe

Introduction: Cell-Based Assays for High-Throughput Screening.- High-Throughput Screening of Model Bacteria.- Cell-Based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells.- Large-Scale Small-Molecule Screen Using Zebrafish Embryos.- Whole-Animal High-Throughput Screens: The C. elegans Model.- Whole-Organism Screening: Plants.- Fluorescence-Based Assays.- Reporter Gene Assays.- Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-Restoration Assay.- Assay for Isolation of Inhibitors of Her2-Kinase Expression.- High-Content Screening: Flow Cytometry Analysis.- High-Throughput Real-Time PCR for Detection of Gene-Expression Levels.- Interpretation of Uniform-Well Readouts.- Extracting Rich Information from Images.

Rezensionen

From the reviews:

"'Cell-based assays for high-throughput screening' is a highly practical resource, written by experts in each of the techniques ... . very useful from a practical point of view, and anyone looking to find a new assay system will find a wealth of ideas here. ... aimed at molecular biologists, many other scientists - such as natural product chemists looking for new lead molecules - should find it equally useful." (Elizabeth Williamson, Phytotherapy Research, September, 2009)