Cell Culture Models of Biological Barriers

In vitro Test Systems for Drug Absorption and Delivery
Herausgeber: Lehr, Claus-Michael

• Gebundenes Buch

Produktbeschreibung

Over the past ten years several sophisticated in vitro test systems based on epithelial cell cultures have been introduced in the field of drug delivery. These models have been found to be very useful in characterizing the permeability of drugs across epithelial tissues, and in studying formulations or carrier systems for improved drug delivery and enhanced absorption. Compared to in vivo trials on animals or humans, cell culture models are faster, more convenient and cost effective, ethically advantageous and, most importantly, they can be more easily standardized and validated. This book provides a practical approach to contemporary cell culture-based in vitro techniques for drug transport studies at biological absorption barriers. It is an invaluable source of information for students attending graduate courses on this subject and pharmaceutical scientists working in industry or in academia.

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Produktdetails

• Verlag: CRC Press
• Seitenzahl: 472
• Erscheinungstermin: 8. August 2002
• Englisch
• Abmessung: 260mm x 183mm x 30mm
• Gewicht: 1077g
• ISBN-13: 9780415277242
• ISBN-10: 0415277248
• Artikelnr.: 22121298

Autorenporträt

Claus-Michael Lehr

Inhaltsangabe

General Aspects of Epithelial Cell and Tissue Culture. Basic Aspects of
Cell Growth and Cell Cycle in Culture. Cell Culture Media: Selection and
Standardization. Bioelectrical Characterization of Epithelial Cell
(Mono)Layers and Tissues. Characterization of Transport Over Epithelial
Barriers. Studying Cellular Binding and Uptake of Bioadhesive Lectins.
High-throughput Epithelial Cell Culture Systems for Screening Drug
Intestinal Permeability. Good Cell Culture Practice (GCCP). Regulatory
Acceptance of in vitro Test Systems as an Alternative to Safety Testing in
Animals. Regulatory Acceptance of in vitro Permeability Studies in the
Context of the Biopharmaceutics Classification System. Models of Specific
Epithelial and Endothelial Barriers Relevant to Drug Delivery. Caco-2 Cell
Monolayers as a Model for Studies of Drug Transport Across Human Intestinal
Epithelium. Transport Studies Using Intestinal Tissue ex vivo. Models of
Alveolar Epithelium. Bronchial Epithelial Cell Cultures. In vitro
Methodologies to Study Nasal Delivery Using Excised Mucosa. Cell Culture
Models of the Corneal and Conjunctival Epithelium. Cell Cultures of the
Retinal Pigment Epithelium to Model the Blood-retina Barrier for Retinal
Drug and Gene Delivery. Human Skin and Skin Equivalents to Study Dermal
Penetration and Permeation. In vitro Models of the Human Buccal Epithelium:
the TR146 Cell Culture Model and the Porcine in vitro Model. Drug Transport
Across the Blood-brain Barrier: A Molecular and Functional Perspective.
BeWo Cells: An in vitro System Representing The Blood-placental Barrier.
Emerging Tools for Studying Biological Barriers and Drug Transport.
Predicting Drug Absorption by Computational Methods. Confocal and
Two-photon Fluorescence Microscopy. On the Application of Scanning Force
Microscopy in Cell Biology. Fluorescence Correlation Spectroscopy.