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The present write up gives a insight on Harvesting of Cells, Cell killing curve, Extraction of protein from eukaryotes, Estimation of protein, SDS-PAGE, 2DE, LC-MS, Mass Spectrometry, extraction and purification RNA, genomic DNA, RNA, Agarose gel electrophoresis of DNA and Extraction from Agarose Gel, DNA transformation, ELISA, Staining includes Monochrome, Negative, and Grams staining of microorganism