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The analysis of recent scientific information on the need to assess, using in vitro or in vivo tests, the toxicity of endogenous and / or exogenous substances has been carried out more specifically in three areas of toxicology, namely genotoxicity, cytotoxicity and endocrine disruption. This summary made it possible to better identify the technical difficulties associated with carrying out toxicological tests, the migration representing only small quantities of products, unidentified or partially identified. Regarding the evaluation of cytotoxicity, a battery of tests seems essential, in order to cover different mechanisms of action (cell survival or development, functional, morphological alterations, etc.).This book is intended more for students and scientists involved in the field of toxicology in order to better understand and for a good mastery of the mode of action of toxic metabolites at the level of cellular organelles, as well as the various tests applied to confirm cytotoxicity. xenobiotics.