Cereal Genomics

Methods and Protocols
Herausgegeben:Vaschetto, Luis M.

• Broschiertes Buch

Produktbeschreibung

The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field.

Produktdetails

• Methods in Molecular Biology 2072
• Verlag: Humana / Springer US / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-9867-8
• 1st ed. 2020
• Seitenzahl: 260
• Erscheinungstermin: 20. September 2020
• Englisch
• Abmessung: 254mm x 178mm x 15mm
• Gewicht: 496g
• ISBN-13: 9781493998678
• ISBN-10: 1493998676
• Artikelnr.: 60044467

Inhaltsangabe

A Low-Cost High-throughput Method for Plant Genomic DNA Isolation.- Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement.- Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping.- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops.- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing.- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR.- DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics.- Genome-wide Identification of Regulatory DNA Elements in Crop Plants.- Genome-wide Profiling of Histone Modifications with ChIP-seq.- Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes.- Genome-wide Identification of Allele-Specific Gene Expression in a Parent of Origin Specific Manner.- Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP).- Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis.- Emerging Genome Engineering Tools in Crop Research and Breeding.- CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids.- Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne.- Agrobacterium Transformation in the Rice Genome.- In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C₄ Photosynthesis in Zea mays.