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Nonfermenting Gram negative bacteria are the potential microbes that are distributed widely in nature and have been isolated from soil, water, and from medical devices as well as from clinical specimens. The Nonfermentative Gram negative bacteria are primarily opportunistic. The (NFGNB) Nonfermentative Gram negative bacteria are known to account for about 15% of all bacterial isolates from a clinical microbiology laboratory. Antibiotic resistance among bacteria is becoming more and more serious problem throughout the world. It is said that evolution of bacteria towards resistance to antimicrobial drugs, including multidrug resistance, is unavoidable because it represents a particular aspect of the general evolution of bacteria that is un-stoppable. Antibiotic resistance emerges commonly when patients are treated with empiric antimicrobial drugs.