Adepu Kiran Kumar

Characteristics and Applications of a Novel Alcohol Oxidase

A highly aggregating broad substrate specific alcohol oxidase protein from petroleum degrading Aspergillus terreus

• Broschiertes Buch

Produktbeschreibung

A microsomal membrane bound alcohol oxidase enzyme was isolated from a hydrocarbon degrading Aspergillus terreus fungus that could oxidize short chain-,long chain-, secondary-, and aryl-alcohol substrates. High aggregating property of the protein was demonstrated by AFM, DLS and TEM analyses. Chemical analysis showed the presence of oleic acid and palmitic acid at a ratio of 2:1 in the purified protein. We have demonstrated a highly efficient method for dissociation and simultaneous deflavination of the alcohol oxidase protein using beta-ME. The potential applications of this approach on preparing apoprotein and its reassemble with FAD to a functional enzyme are envisaged in the preparation of enzyme electrodes for biosensor and biofuel cell applications. The findings obtained through this investigation have revealed many interesting facts about this novel microsomal alcohol oxidase enzyme.This investigation has opened up new avenue on the research to explore the architecture of alcohol oxidase proteins from fungal sources including their structure-function relationship and potential applications.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 152
• Erscheinungstermin: 15. April 2011
• Englisch
• Abmessung: 220mm x 150mm x 10mm
• Gewicht: 244g
• ISBN-13: 9783844331875
• ISBN-10: 3844331875
• Artikelnr.: 33317273

Autorenporträt

Sono un biochimico delle proteine e un biologo strutturale che esplora nuove funzioni delle proteine Post Doctoral Scientist alla Pennsylvania State University, USA PhD in Biotecnologia dall'Indian Institute of Technology, Guwahati, India Master in Biochimica dalla Kakatiya University, Warangal Gold medal Award in Master e Bachelors Degree.