CHARACTERIZE MAMMALIAN TRANSCRIPTOME COMPLEXITY - Wu, Jiaqian
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In my Ph.D. thesis, I described a large scale RT-PCR and cloning pipeline that I developed to obtain several thousand full-length mammalian (mainly human and mouse) cDNA clones for the NHGRI/NCI Mammalian Gene Collection effort. At that time, the layers of the mammalian transcriptome complexity were not yet fully conceived. In this project, I was deeply involved in strategic and conceptual issues in characterizing transcriptome complexity from different levels. Using the RT-PCR pipeline combined with gene prediction algorithms that utilize comparative genomic information, we successfully…mehr

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Produktbeschreibung
In my Ph.D. thesis, I described a large scale RT-PCR and cloning pipeline that I developed to obtain several thousand full-length mammalian (mainly human and mouse) cDNA clones for the NHGRI/NCI Mammalian Gene Collection effort. At that time, the layers of the mammalian transcriptome complexity were not yet fully conceived. In this project, I was deeply involved in strategic and conceptual issues in characterizing transcriptome complexity from different levels. Using the RT-PCR pipeline combined with gene prediction algorithms that utilize comparative genomic information, we successfully identified and experimentally verified novel mammalian genes such as full-length rat homologs of human disease genes. Furthermore, I studied transcriptome complexity including SNPs and alternative splicing in leukemia samples using quantitative PCR, and we developed a trace analysis technology to identify multiple alternative spliced transcripts in one single sequencing reaction. My results suggested that alternatively spliced transcripts show cancer type specific up- or down- regulation, and the ratio of alternative spliced isoforms may underlie the cancer mechanism.
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Autorenporträt
El Dr. Jiaqian Wu es autor de muchas publicaciones revisadas por colegas en el campo de la genética, la genómica y la investigación con células madre. Ganó varios premios y tiene una patente. Jiaqian ha dado charlas en conferencias internacionales e institutos de investigación. Actualmente, Jiaqian es profesor adjunto en la Facultad de Medicina de la Universidad de Texas en Houston.