Frederick J. De Serres, A. Hollaender

Chemical Mutagens

Principles and Methods for Their Detection Volume 8

• Broschiertes Buch

Produktbeschreibung

Volume 8 of Chemical Mutagens covers a wide range of topics in this continuously changing field. This volume includes chapters on the detection of genetic damage in mammalian sperm both at specific loci and over the entire genome. The discussion of in vitro techniques for working with mammalian cells covers not only specific locus assays but also cellular activation systems. Another chapter extensively discusses the need for a revised protocol for the micronucleus assay. Structure activity relationships are investigated in a chapter dealing with hair dye constituents. One of the most comprehensive chapters deals with problems associated with the detection of mutagenic effects in defined human populations. Finally, there is a detailed presentation of a comprehensive study tabulating the genetic bioassay data on some known or suspected human carcinogens. In keeping with our policy of publishing important legislation in the area of chemical mutagens, we have also included the Council of the European Communities Directive of 18 September 1979. Frederick J. de Serres Research Triangle Park, North Carolina vii Contents Chapter 1 Detection of Effects of Mutagens in Human Populations George R. Hoffmann 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2. Monitoring Progeny for Evidence of Germ-Cell Mutations. . . . . 3 2. 1. The Classical Approach: Phenotypic Monitoring . . . . . . . 3 2. 2. Monitoring for Changes in Gene Products . . . . . . . . . . . 7 3. Detection of Gene Mutations in Somatic Cells. . . . . . . . . . . . . 9 3. 1. Drug-Resistant Lymphocytes . . . . . . . . . . . . . . . . . . . . . . 9 3. 2. Hemoglobin Variants . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 4. Nongenetic Indicators of Mutagen Exposure .. . . . . . . . . . . . 21 4. 1. Alkylation of Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 4. 2. DNA Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. .

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Produktdetails

• Verlag: Springer / Springer US / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4613-3696-9
• Softcover reprint of the original 1st ed. 1983
• Seitenzahl: 404
• Erscheinungstermin: 30. November 2011
• Englisch
• Abmessung: 229mm x 152mm x 22mm
• Gewicht: 583g
• ISBN-13: 9781461336969
• ISBN-10: 1461336961
• Artikelnr.: 39511005

Inhaltsangabe

1 Detection of Effects of Mutagens in Human Populations.- 1. Introduction.- 2. Monitoring Progeny for Evidence of Germ-Cell Mutations.- 3. Detection of Gene Mutations in Somatic Cells.- 4. Nongenetic Indicators of Mutagen Exposure.- 5. Testing for Mutagenic Substances in Body Fluids.- 6. Detection of Altered Sperm.- 7. Summary.- 8. References.- 2 The Use of Intact Cellular Activation Systems in Genetic Toxicology Assays.- 1. Introduction.- 2. Mammalian Cell Mutagenesis.- 3. Bacterial Mutagenesis.- 4. Sister Chromatid Exchange and Chromosome Aberrations.- 5. Cell-Mediated Transformation.- 6. Cytotoxicity and Nucleic Acid Interactions.- 7. Discussion.- 8. Summary.- 9. References.- 3 The Mouse Spot Test As a Predictor of Heritable Genetic Damage and Other Endpoints.- 1. Introduction.- 2. Relation to Measures of Heritable Mutations.- 3. Relation to Other Endpoints.- 4. Summary.- 5. References.- 4 The Bone Marrow Micronucleus Assay: Rationale for a Revised Protocol.- 1. Introduction.- 2. Kinetics of Erythropoiesis.- 3. Rationale for the Basic Aspects of the Protocol.- 4. Protocol.- 5. Statistical Analysis.- 6. Conclusions.- 7. References.- 5 Relationships between the Chemical Structure and Mutagenic Activity of Monocyclic Aromatic Amines.- 1. Introduction.- 2. Bacterial Strains.- 3. Experimental Methods.- 4. Chemicals.- 5. Mutagenicity Data and Their Interpretation.- 6. Possible Pitfalls in Studies of Structure-Activity Relationships.- 7. Conclusions.- 8. References.- 6 The Measurement of Recessive Lethal Mutations in the Mouse.- 1. Introduction.- 2. Haldane's Method of Detecting Lethals.- 3. The Backcross Method of Detecting Lethals.- 4. Irradiation Treatment of Male Germ Cells.- 5. Irradiation Treatment of Female Germ Cells.- 6. Chemical Mutagen Induction of RecessiveLethal Mutations.- 7. Dominant Effects of Recessive Lethal Mutations.- 8. Conclusions.- 9. References.- 7 Chemically Induced Changes in Sperm in Animals and Humans.- 1. Introduction.- 2. The F0 Mouse Sperm Head Morphology Assay.- 3. The F1 Sperm Head Morphology Assay.- 4. Induced Sperm Abnormalities in Humans.- 5. Implications of Alterations in Human Sperm Quality.- 6. The Advantages of Sperm Assays.- 7. Recommendations for Future Research and Development.- 8. References.- 8 Mutational Analysis in Cultured Human-Hamster Hybrid Cells.- 1. Introduction.- 2. Origin of Specific Lethal Antisera and Human-Hamster Hybrid Cells.- 3. Characterization of the AL Hybrid.- 4. Mutation Studies.- 5. Conclusions.- 6. References.- 9 Genetic Toxicology of Some Known or Suspected Human Carcinogens.- 1. Introduction.- 2. Chemical Selection.- 3. Method of Literature Review.- 4. Results.- 5. Discussion.- 6. Summary and Conclusions.- 7. Appendix: Complete Data Base.- 8. References.- 10 Control of Commercial Chemicals: The Sixth Amendment to the Directive on Dangerous Chemical Substances (79/83 I/EEC) Adopted by the Council of the European Communities.- 1. Introduction.- 2. Text of the Sixth Amendment to the Directive on Dangerous Chemical Substances Adopted by the Council of the European Communities.- 3. References.

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1 Detection of Effects of Mutagens in Human Populations.
1. Introduction.
2. Monitoring Progeny for Evidence of Germ
Cell Mutations.
3. Detection of Gene Mutations in Somatic Cells.
4. Nongenetic Indicators of Mutagen Exposure.
5. Testing for Mutagenic Substances in Body Fluids.
6. Detection of Altered Sperm.
7. Summary.
8. References.
2 The Use of Intact Cellular Activation Systems in Genetic Toxicology Assays.
1. Introduction.
2. Mammalian Cell Mutagenesis.
3. Bacterial Mutagenesis.
4. Sister Chromatid Exchange and Chromosome Aberrations.
5. Cell
Mediated Transformation.
6. Cytotoxicity and Nucleic Acid Interactions.
7. Discussion.
8. Summary.
9. References.
3 The Mouse Spot Test As a Predictor of Heritable Genetic Damage and Other Endpoints.
1. Introduction.
2. Relation to Measures of Heritable Mutations.
3. Relation to Other Endpoints.
4. Summary.
5. References.
4 The Bone Marrow Micronucleus Assay: Rationale for a Revised Protocol.
1. Introduction.
2. Kinetics of Erythropoiesis.
3. Rationale for the Basic Aspects of the Protocol.
4. Protocol.
5. Statistical Analysis.
6. Conclusions.
7. References.
5 Relationships between the Chemical Structure and Mutagenic Activity of Monocyclic Aromatic Amines.
1. Introduction.
2. Bacterial Strains.
3. Experimental Methods.
4. Chemicals.
5. Mutagenicity Data and Their Interpretation.
6. Possible Pitfalls in Studies of Structure
Activity Relationships.
7. Conclusions.
8. References.
6 The Measurement of Recessive Lethal Mutations in the Mouse.
1. Introduction.
2. Haldane's Method of Detecting Lethals.
3. The Backcross Method of Detecting Lethals.
4. Irradiation Treatment of Male Germ Cells.
5. Irradiation Treatment of Female Germ Cells.
6. Chemical Mutagen Induction of RecessiveLethal Mutations.
7. Dominant Effects of Recessive Lethal Mutations.
8. Conclusions.
9. References.
7 Chemically Induced Changes in Sperm in Animals and Humans.
1. Introduction.
2. The F0 Mouse Sperm Head Morphology Assay.
3. The F1 Sperm Head Morphology Assay.
4. Induced Sperm Abnormalities in Humans.
5. Implications of Alterations in Human Sperm Quality.
6. The Advantages of Sperm Assays.
7. Recommendations for Future Research and Development.
8. References.
8 Mutational Analysis in Cultured Human
Hamster Hybrid Cells.
1. Introduction.
2. Origin of Specific Lethal Antisera and Human
Hamster Hybrid Cells.
3. Characterization of the AL Hybrid.
4. Mutation Studies.
5. Conclusions.
6. References.
9 Genetic Toxicology of Some Known or Suspected Human Carcinogens.
1. Introduction.
2. Chemical Selection.
3. Method of Literature Review.
4. Results.
5. Discussion.
6. Summary and Conclusions.
7. Appendix: Complete Data Base.
8. References.
10 Control of Commercial Chemicals: The Sixth Amendment to the Directive on Dangerous Chemical Substances (79/83 I/EEC) Adopted by the Council of the European Communities.
1. Introduction.
2. Text of the Sixth Amendment to the Directive on Dangerous Chemical Substances Adopted by the Council of the European Communities.
3. References.