An important way to fight against the new borne diseases is by developing new medicines and finding medicinal sources. Hexagonia tenuis (Hook.) Fr., is a least explored and potential bracket fungus. Chemical profiling of H. tenuis revealed the presence of 37 compounds. Few of these were with anti tumor and anti oxidant properties. Because of the presence of number of bioactive components, cytotoxic studies were also carried out and it resulted in a wide spectrum of chromosomal abnormalities. This can be considered as a stepping stone for the further detailed studies in animal assays which may leads to the production of potential mycopharmaceuticals.