Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

Herausgegeben:Chenoweth, David M.

• Gebundenes Buch

Produktbeschreibung

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more.

Produktdetails

• Methods in Enzymology Volume 639
• Verlag: Academic Press / Elsevier Science & Technology
• Artikelnr. des Verlages: S0076-6879(20)X0011-5
• Englisch
• Abmessung: 23mm x 152mm x 229mm
• Gewicht: 770g
• ISBN-13: 9780128211519
• Artikelnr.: 67578425

Autorenporträt

David Chenoweth is at University of Pennsylvania, PA, USA

Inhaltsangabe

1. Fluorogenic detection of protein aggregates in live cells using the AggTag method


Kwan Ho Jung and Xin Zhang




2. Synthesis and application of a ratiometric probe for hydrogen peroxide


Ruwen Yin, Yuan Fang, Xinqi Zhou and Cliff I. Stains




3. Protein labeling for FRET with methoxycoumarin and acridonylalanine


Chloe M. Jones, Yarra Venkatesh and E. James Petersson




4. Ascertaining the activity and inhibition of adenosine deaminase via fluorescence-based assays


Paul T. Ludford III and Yitzhak Tor




5. Method to discriminate amyloids using fluorescent probes


Kristine L. Teppang, Rachel S. Ehrlich and Jerry Yang




6. Chemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivatives


Hui-Hsien Lin, Kelly L. Robertson, Sujeewa S. Lellupitiyage Don, Stephanie R. Taylor and Michelle E. Farkas




7. Methods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancer


John C. Widen, Martina Tholen, Joshua J. Yim and Matthew Bogyo




8. Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles


Nanxi Wang and Lei Wang




9. 4-Cyanoindole-based fluorophores for biological spectroscopy and microscopy


Arusha Acharyya, Ismail A. Ahmed and Feng Gai




10. Dissecting contributions of catalytic and reader domains in regulation of histone demethylation


Nektaria Petronikolou, James E. Longbotham and Danica Galonic Fujimori


11. Ortho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclization


Qing Zhang, Yue Zhang and Xuechen Li




12. Chemical synthesis of di-ubiquitin modified histones for further biochemical studies


Jia-Bin Li, Jun Liang and Changlin Tian




13. Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies


Swathi Merugu, Sahil Sharma, Justin Kaner, Chander Digwal, Mayumi Sugita, Suhasini Joshi, Tony Taldone, Monica L. Guzman and Gabriela Chiosis




14. FlICk (fluorescent isoindole crosslinking) for peptide stapling


Mihajlo Todorovic and David M. Perrin




15. Selective protein N-terminal labeling with N-hydroxysuccinimide esters


Hanjie Jiang, Gabriel D. D'Agostino, Philip A. Cole and Daniel R. Dempsey




16. Covalent live-cell labeling of proteins using a photoreactive fluorogen


Tewoderos M. Ayele, Steve D. Knutson and Jennifer M. Heemstra




17. Photoconvertible diazaxanthilidene dyes for live cell imaging


Rachel M. Lackner, Christopher L. Johnny and David M. Chenoweth




18. No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag

Wei Sheng, James H. Geiger and Babak Borhan