Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems

Herausgegeben:Chenoweth, David M.

• Gebundenes Buch

Produktbeschreibung

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more.

Produktdetails

• Methods in Enzymology Volume 638
• Verlag: Academic Press / Elsevier Science & Technology
• Artikelnr. des Verlages: S0076-6879(20)X0010-3
• Englisch
• Abmessung: 20mm x 152mm x 229mm
• Gewicht: 700g
• ISBN-13: 9780128201459
• Artikelnr.: 67579086

Autorenporträt

David Chenoweth is at University of Pennsylvania, PA, USA

Inhaltsangabe

1. Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates


Ophelia I. Ukaegbu, Kristen E. DeMeester, Hai Liang, Ashley R. Brown, Zachary S. Jones and Catherine Leimkuhler Grimes




2. Chemical tools for selective activity profiling of bacterial penicillin-binding proteins


Shabnam Sharifzadeh, Nathaniel W. Brown, Joshua D. Shirley, Kevin E. Bruce, Malcolm E. Winkler and Erin E. Carlson




3. Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinking


Samir Gautam, Taehan Kim, Rebecca Howell and David A. Spiegel




4. Gram-scale preparation of the antibiotic lead compound salicyl-AMS, a potent inhibitor of bacterial salicylate adenylation enzymes


Nihar Kinarivala, Lisa Standke, Tezcan Guney, Cheng Ji, Naoyoshi Noguchi, Yasutomi Asano and Derek S. Tan




5. Probe-enabled approaches for function-dependent cell sorting and characterization of microbiome subpopulations


Andrea K. Steiger, Sarah J. Fansler, Christopher Whidbey and Aaron T. Wright




6. Biochemical analysis of NlpC/p60 peptidoglycan hydrolase activity


Byungchul Kim, Juliel Espinosa and Howard C. Hang




7. Controlled release of bioactive signaling molecules using DNA-icosahedron


Maulik S. Jani, Aneesh T. Veetil and Yamuna Krishnan




8. Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activation


Hui Chen and Matthew C. Good




9. Engineering reversible cell-cell interactions with chemical biology


Clifford M. Csizmar and Carston R. Wagner




10. Fast phosphine-activated control of protein function using unnatural lysine analogues


Joshua S. Wesalo and Alexander Deiters




11. Photopharmacological control of lipid function


Johannes Morstein and Dirk Trauner




12. Expanding the substrate selectivity in SNAP/CLIP-tagging of intracellular targets


Miguel Macias-Contreras, Kevin N. Little and Lei Zhu




13. Light-induced protein proximity by activation of gibberellic acid derivatives in living cells


Michael J. Ziegler and Richard Wombacher




14. Photoactivatable trimethoprim-based probes for spatiotemporal control of biological processes


Daniel Z. Wu, Michael A. Lampson and David M. Chenoweth




15. Site-specific antibody fragment conjugates for targeted imaging


Robert Maloney, Zakey Yusuf Buuh, Yue Zhao and Rongsheng E. Wang




16. Quantifying protein-protein interactions of the acyl carrier protein with solvatochromic probes

Katherine Charov and Michael D. Burkart