Main description:
Chemokines are major mediators of immune cells and are involved in a wide range of proinflammatory human diseases, including rheumatoid arthritis, multiple sclerosis, and organ transplant rejection. It has recently been discovered that their receptors are involved in HIV infection. The characterization of these molecules and their receptors is thus of primary importance in understanding a number of human diseases and infections. This volume and its companion Volume 287 Chemokines provide comprehensive experimental protocols used in this field of research.
Key - Chemokine receptors
- Chemokines in disease
- Signal transduction of chemokines
Review quote:
Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY
Table of contents:
Chemokine Receptors:
A. Richmond, S. Mueller, J.R. White, and W. Schraw, C-X-C Chemokine Receptor Desensitization Mediated through Ligand-Enhanced Receptor Phosphorylation on Serine Residues.
A. Chuntharapai and K.J. Kim, Generation of Monoclonal Antibodies to Chemokine Receptors.
M. Halks-Miller, J. Hesselgesser, I.J. Miko, and R. Horuk, Chemokine Receptors in Developing Human Brain.
Z-X. Wang, Y-H. Cen, H-H. Guo, J-G. Du, and S.C. Peiper, Expression of Chemokine Receptors in Insect Cells Using Baculovirus Vectors.
S.C. Peiper, Z-H.i Lu, T-Y. Zhang, and Z-X. Wang, Chimeric Chemokine Receptors for Analysis of Structure-Function Relationships.
F.S. Monteclaro, H. Arai, and I.F. Charo, Molecular Approaches to Identifying Ligand Binding and Signaling Domains of C-C Chemokine Receptors.
S. Pitchford, M. Hirst, H.G. Wada, S.D.H. Chan, V.E. Timmermans, and G.M.K. Humphries, Characterization of Functional Activity of Chemokine Receptors Using Cytosensor Microphysiometer. .
P.M. Murphy, Calcium Flux Assay of Chemokine Receptor Expression in Xenopus Oocytes.
J.Rucker, B.J. Doranz, A.L. Edinger, D. Long, J.F. Berson, and R.W. Doms, Cell-Cell Fusion Assay to Study Role of Chemokine Receptors in Human Immunodeficiency Virus Type 1 Entry.
G.L. Bennett and R. Horuk, Iodination of Chemokines for Use in Receptor Binding Analysis.
V. Fingar, H-H. Guo, Z-H. Lu, and S.C. Peiper, Expression of Chemokine Receptors by Endothelial Cells: Detection by Intravital Microscopy Using Chemokine-Coated Fluorescent Microspheres.
Chemokine in Disease:
V.C. Broaddus and C.A. Hebert, Neutralization of Interleukin-8 in in Vivo Models of Lung and Pleural Injury.
A.R. Glabinski, M. Tani, V.K. Tuohy, and R.M. Ransohoff, Murine Experimental Autoimmune Encephalomyelitis: A Model of Immune-Mediated Inflammation and Multiple Sclerosis.
D.A Arenberg, P.J. Polverini, S.L. Kunkel, A. Shanafelt, and R. Strieter, In Vitro and in Vivo Systems to Assess Role of C-X-C Chemokines in Regulation of Angiogenesis.
T.J. Standiford, S.L. Kunkel, and R.M. Strieter, Role of Chemokines in Antibacterial Host Defense.
D.A. Griffiths-Johnson, P.D. Collins, P.J. Jose, and T.J. Williams, Animal Models of Asthma: Role of Chemokines.
J.M. Schrider, Identification and Structural Characterization of Chemokines in Lesional Skin Material of Patients with Inflammatory Skin Disease.
Signal Transduction and Chemokines:
S.R. McColl and P. Naccache, Calcium Mobilization Assays.
S.K. Beckner, G-Protein Activation by Chemokines.
R. Horuk, Adenylate Cyclase Assays to Measure Chemokine Receptor Function.
K.B. Bacon, Analysis of Signal Transduction Following Lymphocyte Activation by Chemokines.
K.B. Bacon, Calcium Mobilization and Phosphoinositide Turnover as Measure of Chemokine Receptor Function in Lymphocytes.
Author Index.
Subject Index.
Chemokines are major mediators of immune cells and are involved in a wide range of proinflammatory human diseases, including rheumatoid arthritis, multiple sclerosis, and organ transplant rejection. It has recently been discovered that their receptors are involved in HIV infection. The characterization of these molecules and their receptors is thus of primary importance in understanding a number of human diseases and infections. This volume and its companion Volume 287 Chemokines provide comprehensive experimental protocols used in this field of research.
Key - Chemokine receptors
- Chemokines in disease
- Signal transduction of chemokines
Review quote:
Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY
Table of contents:
Chemokine Receptors:
A. Richmond, S. Mueller, J.R. White, and W. Schraw, C-X-C Chemokine Receptor Desensitization Mediated through Ligand-Enhanced Receptor Phosphorylation on Serine Residues.
A. Chuntharapai and K.J. Kim, Generation of Monoclonal Antibodies to Chemokine Receptors.
M. Halks-Miller, J. Hesselgesser, I.J. Miko, and R. Horuk, Chemokine Receptors in Developing Human Brain.
Z-X. Wang, Y-H. Cen, H-H. Guo, J-G. Du, and S.C. Peiper, Expression of Chemokine Receptors in Insect Cells Using Baculovirus Vectors.
S.C. Peiper, Z-H.i Lu, T-Y. Zhang, and Z-X. Wang, Chimeric Chemokine Receptors for Analysis of Structure-Function Relationships.
F.S. Monteclaro, H. Arai, and I.F. Charo, Molecular Approaches to Identifying Ligand Binding and Signaling Domains of C-C Chemokine Receptors.
S. Pitchford, M. Hirst, H.G. Wada, S.D.H. Chan, V.E. Timmermans, and G.M.K. Humphries, Characterization of Functional Activity of Chemokine Receptors Using Cytosensor Microphysiometer. .
P.M. Murphy, Calcium Flux Assay of Chemokine Receptor Expression in Xenopus Oocytes.
J.Rucker, B.J. Doranz, A.L. Edinger, D. Long, J.F. Berson, and R.W. Doms, Cell-Cell Fusion Assay to Study Role of Chemokine Receptors in Human Immunodeficiency Virus Type 1 Entry.
G.L. Bennett and R. Horuk, Iodination of Chemokines for Use in Receptor Binding Analysis.
V. Fingar, H-H. Guo, Z-H. Lu, and S.C. Peiper, Expression of Chemokine Receptors by Endothelial Cells: Detection by Intravital Microscopy Using Chemokine-Coated Fluorescent Microspheres.
Chemokine in Disease:
V.C. Broaddus and C.A. Hebert, Neutralization of Interleukin-8 in in Vivo Models of Lung and Pleural Injury.
A.R. Glabinski, M. Tani, V.K. Tuohy, and R.M. Ransohoff, Murine Experimental Autoimmune Encephalomyelitis: A Model of Immune-Mediated Inflammation and Multiple Sclerosis.
D.A Arenberg, P.J. Polverini, S.L. Kunkel, A. Shanafelt, and R. Strieter, In Vitro and in Vivo Systems to Assess Role of C-X-C Chemokines in Regulation of Angiogenesis.
T.J. Standiford, S.L. Kunkel, and R.M. Strieter, Role of Chemokines in Antibacterial Host Defense.
D.A. Griffiths-Johnson, P.D. Collins, P.J. Jose, and T.J. Williams, Animal Models of Asthma: Role of Chemokines.
J.M. Schrider, Identification and Structural Characterization of Chemokines in Lesional Skin Material of Patients with Inflammatory Skin Disease.
Signal Transduction and Chemokines:
S.R. McColl and P. Naccache, Calcium Mobilization Assays.
S.K. Beckner, G-Protein Activation by Chemokines.
R. Horuk, Adenylate Cyclase Assays to Measure Chemokine Receptor Function.
K.B. Bacon, Analysis of Signal Transduction Following Lymphocyte Activation by Chemokines.
K.B. Bacon, Calcium Mobilization and Phosphoinositide Turnover as Measure of Chemokine Receptor Function in Lymphocytes.
Author Index.
Subject Index.