Chemotaxis

Methods and Protocols
Herausgegeben:Jin, Tian; Hereld, Dale

• Gebundenes Buch

Produktbeschreibung

This second edition expands on the first edition with new chapters describing methods for studyingcell movement, molecular components involved in chemotaxis, spatiotemporaldynamics of signaling components, and quantitative modeling, as well as severalupdated chapters from the first edition. Various methods to investigatedirectional cell growth and movements are presented in Chapters 1-20. Thesechapters contains experimental procedures to visualize and measure migrationbehaviors of different kinds of organisms, including chemotropism in thebudding yeast; cell growth and migration of D. discoideum; border cellmigration in Drosophila; chemotaxis of mouse and human neutrophils; andHIV-induced T cell chemotactic response. Chemotaxis: Methods and Protocols,Second Edition also contains microscopy procedures for studying breast cancercell migration, tumor cell invasion in vivo, and axon guidance. The book concludeswith Chapters 21-27 describing methods that measure spatiotemporal dynamics ofsignaling components involved in chemotaxis; introduce imaging techniques, suchas TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models ofexperimentally generated chemoattractant gradients. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.

Cutting edge and thorough,Chemotaxis: Methods and Protocols, Second Edition is a valuable resource foranyone who is interested in the diverse methodologies that are propellingchemotaxis research forward.

Produktdetails

• Methods in Molecular Biology 1407
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-3478-2
• 2. Aufl.
• Seitenzahl: 440
• Erscheinungstermin: 7. Juni 2016
• Englisch
• Abmessung: 260mm x 183mm x 30mm
• Gewicht: 948g
• ISBN-13: 9781493934782
• ISBN-10: 1493934783
• Artikelnr.: 43903981

Inhaltsangabe

In Situ Assays of Chemotropism duringYeast Mating.- ImagingPolarization in Budding Yeast Migration of Dictyostelium discoideum to theChemoattractant Folic Acid.- MitochondrialStress Tests Using Seahorse Respirometry on Intact Dictyostelium discoideum Cells.- StudyingChemoattractant Signal Transduction Dynamics in Dictyostelium by BRET.- Wave Patternsin Cell Membrane and Actin Cortex Uncoupled from Chemotactic Signals.- ChemotacticBlebbing in Dictyostelium Cells.- DissectingSpatial and Temporal Sensing in DictyosteliumChemotaxis Using a Wave Gradient Generator.- Employing Dictyostelium as an Advantageous 3RsModel for Pharmacogenetic Research.- Identificationof Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis.- BiochemicalResponses to Chemically Distinct Chemoattractants during the Growth andDevelopment of Dictyostelium.- Live Imaging ofBorder Cell Migrationin Drosophila.- ShRNA-InducedGene Knock-Down In Vivo to Investigate Neutrophil Function.- StudyingNeutrophil Migration In Vivo Using Adoptive Cell Transfer.- IntravitalTwo-Photon Imaging of Lymphocytes Crossing High Endothelial Venules andCortical Lymphatics in the Inguinal Lymph Node.- FlowCytometry-Based Quantification of HIV-Induced T Cell Chemotactic Response.- VisualizingCancer Cell Chemotaxis and Invasion in 2D and 3D.- 4D TumorigenesisModel for Quantitating Coalescence, Directed Cell Motility and Chemotaxis,Identifying Unique Cell Behaviors and Testing Anti-Cancer Drugs.- An ExperimentalModel for Simultaneous Study of Migration of Cell Fragments, Single Cells andCell Sheets.- Axon GuidanceStudies Using a Microfluidics-Based Chemotropic Gradient Generator.- Visualization ofActin Assembly and Filament Turnover by In Vitro Multi-Color TIRF Microscopy.- QuantitativeMonitoring Spatiotemporal Activation of Ras and PKD1 Using Confocal FluorescentMicroscopy.- FluorescenceReadout of a Patch Clamped Membrane by Laser Scanning Microscopy.- Use of ResonanceEnergy Transfer Techniques for In Vivo Detection of Chemokine ReceptorOligomerization.- Multi-StateTransition Kinetics of Intracellular Signaling Molecules by Single-MoleculeImaging Analysis.- Mathematics ofExperimentally Generated Chemoattractant Gradients.- ModelingExcitable Dynamics of Chemotactic Networks.