Cilia

Methods and Protocols
Herausgegeben:Satir, Peter; Christensen, Søren Tvorup

• Gebundenes Buch

Produktbeschreibung

This volume is the result of an explosion ofmolecular-based research on Cilia, which began with the discovery of theuniversality of intraflagellar transport (IFT) and ciliary genomics/proteomics.The chapters in this book cover topics such as: high resolution imaging andfunctional characterization of sensory and primary cilia in mammalian cells andzebrafish, methods to study ciliary-mediated chemoresponse in Paramecium,and methods to study centrosomes and cilia in C. elegans and Drosophila.Written in the highly successful Methods in Molecular Biology series format,chapters include introduction to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Cilia:Methods and Protocols is broad and covers motile, sensory, and primarycells. It is a valuable resource to anyone interested in entering the field ofciliary biology using model organisms, including flagellate algae, ciliates,planaria, nematodes, insects, zebrafish, and mammalian cells.

Produktdetails

• Methods in Molecular Biology 1454
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-3787-5
• 1st ed. 2016
• Seitenzahl: 276
• Erscheinungstermin: 12. August 2016
• Englisch
• Abmessung: 260mm x 183mm x 21mm
• Gewicht: 651g
• ISBN-13: 9781493937875
• ISBN-10: 1493937871
• Artikelnr.: 44689054

Inhaltsangabe

Methods for Studying Ciliary Import Mechanisms.- Targeting of ASH Domain-Containing Proteins tothe Centrosome.- Morphological and Functional Characterizationof the Ciliary Pocket by Electron and Fluorescence Microscopy.- Methods to Study Interactions between Ciliogenesisand Autophagy.- Recombinant Reconstitution and Purification ofthe IFT B Core Complex from Chlamydomonasreinhardtii.- Methods for Studying Movement of Moleculeswithin Cilia.- A FRAP-Based Method for Monitoring Molecular Transportin Ciliary Photoreceptor Cells In Vivo.- Kymographic Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans.- Visualization and Manipulation of Cilia and IntraciliaryCalcium in the Zebrafish Left-Right Organizer.- Methods for Studying Ciliary-Mediated Chemoresponsein Paramecium.- STEDand STORM Superresolution Imaging of Primary Cilia.- CLEM Methods forStudying Primary Cilia.- Methods for Visualization of Neuronal Cilia.- Methods to Study Centrosomes and Cilia in Drosophila.- Analysis of Axonemal Assembly during Ciliary Regenerationin Chlamydomonas.- Planaria as aModel System for the Analysis of Ciliary Assembly and Motility.