Ezio Rosato (ed.)

Circadian Rhythms

Methods and Protocols
Herausgegeben:Rosato, Ezio

• Gebundenes Buch

Produktbeschreibung

The aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or undertaking comparative studies and would like to consult protocols applied to different organisms before starting the study of new species. This book features a full range of methods that illustrate procedures that have been recently been introduced in circadian studies and by presenting variations to take into account the peculiarities of different model systems.

---

Hinweis: Dieser Artikel kann nur an eine deutsche Lieferadresse ausgeliefert werden.

Produktdetails

• Methods in Molecular Biology 362
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 12013111, 978-1-58829-417-3
• 2007 edition
• Seitenzahl: 578
• Erscheinungstermin: 2. Februar 2007
• Englisch
• Abmessung: 232mm x 155mm x 36mm
• Gewicht: 1026g
• ISBN-13: 9781588294173
• ISBN-10: 158829417X
• Artikelnr.: 21304334

Inhaltsangabe

Overviews.- Light, Photoreceptors, and Circadian Clocks.- Statistical Analysis of Biological Rhythm Data.- Rhythmic Readouts.- Rhythmic Conidiation in Neurospora crassa.- Monitoring and Analyzing Drosophila Circadian Locomotor Activity.- Automated Video Image Analysis of Larval Zebrafish Locomotor Rhythms.- Locomotor Activity in Rodents.- Analysis of Circadian Leaf Movement Rhythms in Arabidopsis thaliana.- Detection of Rhythmic Bioluminescence From Luciferase Reporters in Cyanobacteria.- Analysis of Rhythmic Gene Expression in Adult Drosophila Using the Firefly Luciferase Reporter Gene.- Monitoring Circadian Rhythms in Arabidopsis thaliana Using Luciferase Reporter Genes.- Identification of Clock Genes.- Specialized Techniques for Site-Directed Mutagenesis in Cyanobacteria.- Novel Strategies for the Identification of Clock Genes Neurospora With Insertional Mutagenesis.- Mutagenesis With Drosophila.- Mutagenesis in Arabidopsis.- Yeast Two-Hybrid Screening.- Microarrays.- Microarrays.- Identification of Clock Genes Using Difference Gel Electrophoresis.- Gene Expression: RNA.- Isolation of Total RNA From Neurospora Mycelium.- RNA Extraction From Drosophila Heads.- Extraction of Plant RNA.- RNA Extraction From Mammalian Tissues.- Northern Analysis of Sense and Antisense frequency RNA in Neurospora crassa.- RNase Protection Assay.- Quantitative Polymerase Chain Reaction.- Gene Expression: Proteins.- Protein Extraction, Fractionation, and Purification From Cyanobacteria.- Protein Extraction From Drosophila Heads.- Plant Protein Extraction.- Protein Extraction From Mammalian Tissues.- Western Blotting.- Coimmunoprecipitation Assay.- In Vitro Phosphorylation and Kinase Assays in Neurospora crassa.- In Vitro Systems.- Basic Protocols for Drosophila S2 Cell Line.- Coimmunoprecipitation on Drosophila Cells in Culture.- Basic Protocols for Zebrafish Cell Lines.- Manipulation of Mammalian Cell Lines for Circadian Studies.- Reporter Assays.- Use of Firefly Luciferase Activity Assays to Monitor Circadian Molecular Rhythms In Vivo and In Vitro.- Suprachiasmatic Nucleus Cultures That Maintain Rhythmic Properties In Vitro.- Microscopy Analysis.- RNA In Situ Hybridizations on Drosophila Whole Mounts.- In Situ Hybridization of Suprachiasmatic Nucleus Slices.- Immunohistochemistry in Drosophila.- Immunocytochemistry on Suprachiasmatic Nucleus Slices.- Immunofluorescence Analysis of Circadian Protein Dynamics in Cultured Mammalian Cells.