Introduces the reader to Circulating Tumor Cells (CTCs), their isolation method and analysis, and commercially available platforms _ Presents the historical perspective and the overview of the field of circulating tumor cells (CTCs) _ Discusses the state-of-art methods for CTC isolation, ranging from the macro- to micro-scale, from positive concentration to negative depletion, and from biological-property-enabled to physical-property-based approaches _ Details commercially available CTC platforms _ Describes post-isolation analysis and clinical translation _ Provides a glossary of scientific terms related to CTCs…mehr
Introduces the reader to Circulating Tumor Cells (CTCs), their isolation method and analysis, and commercially available platforms _ Presents the historical perspective and the overview of the field of circulating tumor cells (CTCs) _ Discusses the state-of-art methods for CTC isolation, ranging from the macro- to micro-scale, from positive concentration to negative depletion, and from biological-property-enabled to physical-property-based approaches _ Details commercially available CTC platforms _ Describes post-isolation analysis and clinical translation _ Provides a glossary of scientific terms related to CTCsHinweis: Dieser Artikel kann nur an eine deutsche Lieferadresse ausgeliefert werden.
Produktdetails
Produktdetails
Chemical Analysis: A Series of Monographs on Analytical Chemistry and Its Applications
Z. Hugh Fan, PhD, is a professor of the Department of Mechanical and Aerospace Engineering, J. Crayton Pruitt Family Department of Biomedical Engineering, and Department of Chemistry at the University of Florida (UF), USA. Prior to joining UF in 2003, Dr. Fan was a Principal Scientist at ACLARA BioSciences Inc. and was previously a Member of the Technical Staff at Sarnoff Corp. Dr. Fan has been recognized with E.T.S. Walton Award from Science Foundation of Ireland in 2009, Fraunhofer-Bessel Research Award from Alexander von Humboldt Foundation (Germany) in 2010, and UF Research Foundation Professorship in 2014.
Inhaltsangabe
List of Contributors xv
Foreword xxi
Preface xxv
PART I INTRODUCTION 1
1 Circulating Tumor Cells and Historic Perspectives 3 Jonathan W. Uhr
1.1 Early Studies on Cancer Dormancy Led to the Development of a Sensitive Assay for CTCs (1970-1998) 3
1.2 Modern Era for Counting CTCs: 1998-2007 6
1.3 Proof of Malignancy of CTCs 7
1.4 New Experiments Involving CTCs 7
1.5 Clinical Cancer Dormancy 8
1.6 Human Epidermal Growth Factor Receptor 2 (HER2) Gene Amplification can be Acquired as Breast Cancer Progresses 10
1.7 uPAR and HER2 Co-amplification 11
1.8 Epithelial-Mesenchymal Transition (EMT) 12
1.9 New Instruments to Capture CTCs 14
1.10 Genotypic Analyses 15
1.11 Conclusions 18
References 20
2 Introduction to Microfluidics 33 Kangfu Chen and Z. Hugh Fan
2.1 Introduction 33
2.2 Scaling Law 36
2.3 Device Fabrication 39
2.4 Functional Components in Microfluidic Devices 43
2.5 Concluding Remarks 46
References 47
PART II ISOLATION METHODS 51
3 Ensemble-decision Aliquot Ranking (eDAR) for CTC Isolation and Analysis 53 Mengxia Zhao, Perry G. Schiro, and Daniel T. Chiu
3.1 Overview of eDAR 53
3.2 Individual Components and Analytical Performance of eDAR 55
3.3 Application and Downstream Analyses of eDAR 69
3.4 Conclusion and Perspective 80
References 81
4 Sinusoidal Microchannels with High Aspect Ratios for CTC Selection and Analysis 85 Joshua M. Jackson, MaBgorzata A. Witek, and Steven A. Soper
4.1 Introduction 85
4.2 Parallel Arrays of High-Aspect-Ratio, Sinusoidal Microchannels for CTC Selection 90
4.3 Clinical Applications of Sinusoidal CTC Microchip 114
4.4 Conclusion 118
Acknowledgments 119
References 119
5 Cell Separation using Inertial Microfluidics 127 Nivedita Nivedita and Ian Papautsky
5.1 Introduction 127
5.2 Device Fabrication and System Setup 128
5.3 Inertial Focusing in Microfluidics 129
5.4 Cancer Cell Separation in Straight Microchannels 132
5.5 Cancer Cell Separation in Spiral Microchannels 136
5.6 Conclusions 142
References 142
6 Morphological Characteristics of CTCs and the Potential for Deformability-Based Separation 147 Simon P. Duffy and Hongshen Ma
6.1 Introduction 147
6.2 Limitations of Antibody-based CTC Separation Methods 148
6.3 Morphological and Biophysical Differences Between CTCs and Hematological Cells 149
6.4 Historical and Recent Methods in CTC Separation Based on Biophysical Properties 153
6.5 Microfluidic Ratchet for Deformability-Based Separation of CTCs 155
6.6 Resettable Cell Trap for Deformability-based Separation of CTCs 160
6.7 Summary 165
References 166
7 Microfabricated Filter Membranes for Capture and Characterization of Circulating Tumor Cells (CTCs) 173 Zheng Ao, Richard J. Cote, Ram H. Datar, and Anthony Williams
7.1 Introduction 173
7.2 Size-based Enrichment of Circulating Tumor Cells 174
7.3 Comparison Between Size-based CTC Isolation and Affinity-based Isolation 177
7.4 Characterization of CTCs Captured by Microfilters 178
7.5 Conclusion 180
References 181
8 Miniaturized Nuclear Magnetic Resonance Platform for Rare Cell Detection and Profiling 183 Sangmoo Jeong, Changwook Min, Huilin Shao, Cesar M. Castro,
1 Circulating Tumor Cells and Historic Perspectives 3 Jonathan W. Uhr
1.1 Early Studies on Cancer Dormancy Led to the Development of a Sensitive Assay for CTCs (1970-1998) 3
1.2 Modern Era for Counting CTCs: 1998-2007 6
1.3 Proof of Malignancy of CTCs 7
1.4 New Experiments Involving CTCs 7
1.5 Clinical Cancer Dormancy 8
1.6 Human Epidermal Growth Factor Receptor 2 (HER2) Gene Amplification can be Acquired as Breast Cancer Progresses 10
1.7 uPAR and HER2 Co-amplification 11
1.8 Epithelial-Mesenchymal Transition (EMT) 12
1.9 New Instruments to Capture CTCs 14
1.10 Genotypic Analyses 15
1.11 Conclusions 18
References 20
2 Introduction to Microfluidics 33 Kangfu Chen and Z. Hugh Fan
2.1 Introduction 33
2.2 Scaling Law 36
2.3 Device Fabrication 39
2.4 Functional Components in Microfluidic Devices 43
2.5 Concluding Remarks 46
References 47
PART II ISOLATION METHODS 51
3 Ensemble-decision Aliquot Ranking (eDAR) for CTC Isolation and Analysis 53 Mengxia Zhao, Perry G. Schiro, and Daniel T. Chiu
3.1 Overview of eDAR 53
3.2 Individual Components and Analytical Performance of eDAR 55
3.3 Application and Downstream Analyses of eDAR 69
3.4 Conclusion and Perspective 80
References 81
4 Sinusoidal Microchannels with High Aspect Ratios for CTC Selection and Analysis 85 Joshua M. Jackson, MaBgorzata A. Witek, and Steven A. Soper
4.1 Introduction 85
4.2 Parallel Arrays of High-Aspect-Ratio, Sinusoidal Microchannels for CTC Selection 90
4.3 Clinical Applications of Sinusoidal CTC Microchip 114
4.4 Conclusion 118
Acknowledgments 119
References 119
5 Cell Separation using Inertial Microfluidics 127 Nivedita Nivedita and Ian Papautsky
5.1 Introduction 127
5.2 Device Fabrication and System Setup 128
5.3 Inertial Focusing in Microfluidics 129
5.4 Cancer Cell Separation in Straight Microchannels 132
5.5 Cancer Cell Separation in Spiral Microchannels 136
5.6 Conclusions 142
References 142
6 Morphological Characteristics of CTCs and the Potential for Deformability-Based Separation 147 Simon P. Duffy and Hongshen Ma
6.1 Introduction 147
6.2 Limitations of Antibody-based CTC Separation Methods 148
6.3 Morphological and Biophysical Differences Between CTCs and Hematological Cells 149
6.4 Historical and Recent Methods in CTC Separation Based on Biophysical Properties 153
6.5 Microfluidic Ratchet for Deformability-Based Separation of CTCs 155
6.6 Resettable Cell Trap for Deformability-based Separation of CTCs 160
6.7 Summary 165
References 166
7 Microfabricated Filter Membranes for Capture and Characterization of Circulating Tumor Cells (CTCs) 173 Zheng Ao, Richard J. Cote, Ram H. Datar, and Anthony Williams
7.1 Introduction 173
7.2 Size-based Enrichment of Circulating Tumor Cells 174
7.3 Comparison Between Size-based CTC Isolation and Affinity-based Isolation 177
7.4 Characterization of CTCs Captured by Microfilters 178
7.5 Conclusion 180
References 181
8 Miniaturized Nuclear Magnetic Resonance Platform for Rare Cell Detection and Profiling 183 Sangmoo Jeong, Changwook Min, Huilin Shao, Cesar M. Castro,
Ralph Weissleder, and Hakho Lee
8.1 Introduct
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