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Cholistan desert is a wild land of its own kind with scarcity of endemic flora, counting only 128 species belonging to 34 families. Homeopathy is a system of therapeutics and 80% homeopathic medicines are based upon plants origin in the form of mother tinctures. Present study was designed to explore homeopathic way of treatment of various diseases by using the mother tincture. A double blind clinical study of mother tinctures and carbo-vegetabilis was performed based on the ethno-botanical information collected from the local people of Cholistan desert. The mother tincture of 19 medicinal plants and carbo-vegetabilis of 4 medicinal plants of Cholistan desert were clinically evaluated against various diseases. The results showed that out of 19 medicinal plants the mother tinctures of 8 plant species was very effective to control various diseases by giving 75-100 % curing percentage. The results of present study concluded that the homeo-mother tinctures of various medicinal plants from Cholistan desert should be trialed and this cheap and safe way of medication should be promoted in the area to control various diseases.