Computational analysis of STMADS11 MADS-box regulatory elements - Hussain, Tajammul;Khan, Muhammad Ramzan
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Functions of orthologs may diverge during evolution either due to alteration of cis-regulatory elements or changes in the coding regions. The STMADS11 superclade MADS-box genes play diverse roles in plants; implicated in vegetative development, phase transition, floral meristem identity, male fertility and inflated calyx. Phylogenetic reconstruction revealed three distinct clades comprising MPF1-like, MPF2-like and SVP-like. Expression analysis indicated inter and intra-clade expression divergence with regard to their expression in vegetative and floral tissues. The MADS-domain HCTs were…mehr

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Produktbeschreibung
Functions of orthologs may diverge during evolution either due to alteration of cis-regulatory elements or changes in the coding regions. The STMADS11 superclade MADS-box genes play diverse roles in plants; implicated in vegetative development, phase transition, floral meristem identity, male fertility and inflated calyx. Phylogenetic reconstruction revealed three distinct clades comprising MPF1-like, MPF2-like and SVP-like. Expression analysis indicated inter and intra-clade expression divergence with regard to their expression in vegetative and floral tissues. The MADS-domain HCTs were predominantly located in the promoter regions. These MADS-domain HCTs are located in the vicinity of the TSS seemed to be involved in regulating expression in sepal, particularly in case of MPF2-like clade. Phylogenetic foot printing of -2kb STMADS11 promoter sequences highlighted two conserve regions; immediately upstream of TSS site and proximal to TSS. These regions have evolved under negativeselection. A comprehensive understanding of the role of STMADS11 cis-regulatory elements in expression divergence is though enigmatic, this study highlights the significance of cis-regulatory elements.
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Autorenporträt
Tajammul Hussain has completed his M.Phil in the field of Plant Genomics & Biotechnology from KPK-AUP Pakistan. Research was carried out at NIGAB under the supervision of DR. MR Khan. Since then he has worked in ¿Genetic Improvement of Groundnut for Herbicide and Disease Resistance¿ and ¿Rapid Identification of Olive Cultivars using DNA markers"