Michael J. Corey

Coupled Bioluminescent Assays

Methods, Evaluations, and Applications

• Gebundenes Buch

Produktbeschreibung

Brings the field of coupled bioluminescence to mainstream science

This book enables readers to take full advantage of coupled bioluminescent (CB) assay techniques by demonstrating how they are used to solve real-world problems. It guides readers involved in drug discovery and biomedicine through a broad range of valuable CB techniques, including assays of kinases, phosphatases, and other enzymes; cytotoxicity measurements; and high-throughput screening of receptor modulators. In each case, CB methods are compared with competing technologies to enable informed choices. In addition, the book's coverage of CB methods in biomonitoring; analysis of nitrate, a critical marker of explosives residue and fertilizer runoff; and nerve agent and pesticide detection supports the work of researchers involved in biodefense as well as environmental and food sciences.

Coupled Bioluminescent Assays is divided into three parts:
Background to coupled bioluminescent assays
Biomedical applications of coupled bioluminescence
Other applications of coupled bioluminescence

Highlights of coverage include:
CB cytotoxicity assays
CB assays of kinases and phosphatases
CB measurements of G protein coupled receptor activity
Assessment of nitric oxide synthase activity by CB
CB determination of bioburden and sterility
Emerging regulatory concerns

Each chapter clearly presents both underlying theory and applications. References facilitate further investigations by guiding readers to the primary literature.

CB techniques offer advantages in speed and sensitivity that fluorometric techniques generally cannot match. Bioluminescent reaction schemes are typically one to several orders of magnitude more sensitive than fluorometric assays, yet these techniques have been vastly underutilized. With this book as their guide, chemists, biochemists, and molecular biologists can take full advantage of a broad range of available CB assays to achieve their research goals.

Produktdetails

• Verlag: Wiley & Sons
• 1. Auflage
• Seitenzahl: 312
• Erscheinungstermin: 1. November 2008
• Englisch
• Abmessung: 240mm x 161mm x 22mm
• Gewicht: 574g
• ISBN-13: 9780470108833
• ISBN-10: 0470108835
• Artikelnr.: 25625358

Autorenporträt

Michael J. Corey, PHD, is a consulting biochemist and a leading authority on coupled bioluminescent assays. A former head of assay development in biotechnology, he is a founding member of the Scientific Advisory Board of Cell Technology, Inc. Dr. Corey holds key patents in the field of coupled bioluminescence.

Inhaltsangabe

PREFACE. PART I BACKGROUND TO COUPLED BIOLUMINESCENT ASSAYS. 1
Introduction. 1.1 Introduction to Coupled Bioluminescent Assays. 1.2
Luminescent Technologies of the Life Sciences. 1.3 Varieties of
Fluorometric Assays. 1.4 Chemiluminescence and Bioluminescence. 1.5 Common
Bioluminescence Systems. 1.6 A Coupled Bioluminescent Reaction. 1.7
Summary. 2 Coupled Bioluminescent Reactions in Practice. 2.1 Principles of
Coupled Bioluminescent Reactions. 2.2 Instrumentation and Equipment for
Coupled Bioluminescent Assays. 2.3 Coupled Bioluminescent Assay Procedures
and Precautions. 2.4 Data Handling for Coupled Bioluminescent Assays. 2.5
Comparison of Coupled Bioluminescent Assays with Other Methods. PART II
BIOMEDICAL APPLICATIONS OF COUPLED BIOLUMINESCENCE. 3 Coupled
Bioluminescent Cytotoxicity Assays. 3.1 Introduction. 3.2 Membrane
Integrity Assays. 3.3 A Coupled Bioluminescent Assay for Enzyme Release:
The G3PDH-Release Assay. 3.4 Viability Assays. 4 The Role of Coupled
Bioluminescent Assays in Kinase Screening and Study. 4.1 The Many Roles of
Kinases in Biology. 4.2 Current Standard Kinase Assays. 4.3 Coupled
Bioluminescent Kinase Assays. 4.4 Conclusions. 5 Coupled Bioluminescent
Phosphatase Assays. 5.1 Introduction. 5.2 Phosphatases. 5.3 Contemporary
Phosphatase Assay Technologies. 5.4 CB Phosphatase Assays. 5.5 Conclusions.
6 Acetylcholinesterase. 6.1 Introduction. 6.2 Established AChE Assay
Methods. 6.3 Recent Developments in AChE Assay Methods. 6.4 Evaluation of
Current AChE High-Throughput Screening Methods. 6.5 Coupled Bioluminescent
Assays of AChE Activity. 6.6 Comparison of Coupled Bioluminescent and Other
Methods of Measuring AChE Activity. 7 Measurement of Nitric Oxide Synthase
Activity by Coupled Bioluminescence. 7.1 Introduction. 7.2 Current NOS
Assays. 7.3 Conclusions. 8 The Coupled Bioluminescent Pyrophosphorolysis
Assay. 8.1 Introduction. 8.2 Genetic Variation in Modern Medicine. 8.3 DNA
Complementarity. 8.4 The READIT(r) Pyrophosphorolysis Assay. 9 Coupled
Luminescent Assays of G-Protein-Coupled Receptors. 9.1 Introduction to
G-Protein-Coupled Receptors. 9.2 GPCR Assay Methods. 9.3 Summary. 10
Coupled Bioluminescent Protease Assays. 10.1 Introduction. 10.2 Protease
Assays. 10.3 Coupled Bioluminescent Protease Assays. 10.4 Summary. 11
Coupled Luminescent Assays Involving Aequorin. 11.1 Introduction to
Aequorin. 11.2 Detection of Calcium in Practice. 12 Coupled Bioluminescent
Reporter Assays. 12.1 Introduction to Reporter Assays. 12.2 Luciferases as
Reporters of Promoter Activities. 12.3 Aequorin as a Reporter Enzyme. 12.4
Vectors for Use in Reporter Assays. 12.5 Summary. 13 Coupled Bioluminescent
Assays: Regulatory Concerns. 13.1 Introduction. 13.2 Regulatory Aspects of
Assay Development. 13.3 Summary. PART III OTHER APPLICATIONS OF COUPLED
BIOLUMINESCENCE. 14 Coupled Bioluminescent Determination of Bioburden and
Sterility. 14.1 Introduction. 14.2 Rapid Methods of Bioburden and Sterility
Assessment. 15 Environmental Applications of Coupled Bioluminescent Assays.
15.1 Introduction. 15.2 Current Methods for Environmental Monitoring of
Water Quality. 15.3 Methods of Monitoring Stream Water and Lake Water. 15.4
Methods of Monitoring Drinking Water. Appendix A: One-Letter Amino Acid
Abbreviations. Glossary. Bibliography. Index.