This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
1. Fundamentals of three-dimensional reconstruction from projections Pawel A. Penczek 2. Image Restoration in Cryo-electron Microscopy Pawel A. Penczek 3. Resolution measures in molecular electron microscopy Pawel A. Penczek 4. 3D reconstruction from 2D crystal image and diffraction data Andreas Schenk, Daniel Castaño-Díez, Bryant Gipson, Marcel Arheit, Xiangyan Zeng, and Henning Stahlberg 5. Fourier-Bessel Reconstruction of Helical Assemblies David L Stokes, Ruben Diaz & William, J. Rice 6. Reconstruction of Helical Filaments and Tubes Edward H Egelman 7. Three-Dimensional Asymmetric Reconstruction of Tailed Bacteriophage Jinghua Tang, Robert S. Sinkovits, and Timothy S. Baker 8. Single Particle Analysis at High Resolution Yao Cong and Steven J. Ludtke 9. The Orthogonal Tilt Reconstruction method Andres Leschziner 10. An introduction to maximum-likelihood methods in cryo-EM Fred J. Sigworth, Peter C. Doerschuk, Jose-Maria Carazo, and Sjors H.W. Scheres 11. Classification of structural heterogeneity by maximum-likelihood methods Sjors H.W. Scheres 12. Methods for three-dimensional reconstruction of heterogeneous assemblies Elena V Orlova, Helen R. Saibil 13. Alignment of cryo-electron tomography datasets Fernando Amat, Daniel Casta~no-Diez, Albert Lawrence, Farshid Moussavi, Hanspeter Winkler, & Mark Horowitz 14. Correcting for the Ewald sphere in high resolution single particle reconstructions Grant J Jensen, Peter A. Leong, Xuekui Yu, & Z. Hong Zhou 15. Software Tools For Molecular Microscopy: An Open-Text Wikibook Bridget Carragher, Neil R. Voss, Clinton S. Potter, & Ross Smith
1. Fundamentals of three-dimensional reconstruction from projections Pawel A. Penczek 2. Image Restoration in Cryo-electron Microscopy Pawel A. Penczek 3. Resolution measures in molecular electron microscopy Pawel A. Penczek 4. 3D reconstruction from 2D crystal image and diffraction data Andreas Schenk, Daniel Castaño-Díez, Bryant Gipson, Marcel Arheit, Xiangyan Zeng, and Henning Stahlberg 5. Fourier-Bessel Reconstruction of Helical Assemblies David L Stokes, Ruben Diaz & William, J. Rice 6. Reconstruction of Helical Filaments and Tubes Edward H Egelman 7. Three-Dimensional Asymmetric Reconstruction of Tailed Bacteriophage Jinghua Tang, Robert S. Sinkovits, and Timothy S. Baker 8. Single Particle Analysis at High Resolution Yao Cong and Steven J. Ludtke 9. The Orthogonal Tilt Reconstruction method Andres Leschziner 10. An introduction to maximum-likelihood methods in cryo-EM Fred J. Sigworth, Peter C. Doerschuk, Jose-Maria Carazo, and Sjors H.W. Scheres 11. Classification of structural heterogeneity by maximum-likelihood methods Sjors H.W. Scheres 12. Methods for three-dimensional reconstruction of heterogeneous assemblies Elena V Orlova, Helen R. Saibil 13. Alignment of cryo-electron tomography datasets Fernando Amat, Daniel Casta~no-Diez, Albert Lawrence, Farshid Moussavi, Hanspeter Winkler, & Mark Horowitz 14. Correcting for the Ewald sphere in high resolution single particle reconstructions Grant J Jensen, Peter A. Leong, Xuekui Yu, & Z. Hong Zhou 15. Software Tools For Molecular Microscopy: An Open-Text Wikibook Bridget Carragher, Neil R. Voss, Clinton S. Potter, & Ross Smith
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