Harry Moore (Hrsg.)

Culture of Human Embryonic Stem Cells: Methods and Techniques

Herausgeber: Moore, Harry

• Broschiertes Buch

Produktbeschreibung

A handbook of the practical aspects of working with human embryonic stem cells from their derivation to the development of clinical applications. The chapters would start with a brief review and then give practical details for experimental analysis and research. It would also cover ethical issues and requirements for clinical developments.

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Hinweis: Dieser Artikel kann nur an eine deutsche Lieferadresse ausgeliefert werden.

Produktdetails

• Stem Cell Biology and Regenera
• Verlag: Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 12226610
• 2012
• Seitenzahl: 250
• Erscheinungstermin: November 2011
• Englisch
• Abmessung: 235mm x 155mm
• ISBN-13: 9781603275392
• ISBN-10: 1603275398
• Artikelnr.: 23801830

Inhaltsangabe

From the contents:
Background to embryonic stem cells.- Regulatory and ethical issues.- Setting up a lab.- Establishing a hES research programme.- Embryonic stem cell derivation.- Principles of hES cell culture.- Cryopreservation of cell lines.- Feeder-free techniques.- Advanced culture techniques for hES cells.- Avoiding culture pitfalls.- Methods of differentiation.- Differentiation Protocols.- Analysis of differentiation.- Immunofluorescent labeling for surface antigens and FACS analysis.- Flow Cytometry.- Transfection.- Molecular profiling of hES cells.- Quality control and assurance.