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Bangladesh is a land of medicinal plants. From previous time to till date medicinal plants are using as a traditional medicine. Research on natural products is now a prime issue. Azadirachta indica roots and leaves are very useful in blood circulation and purification. It is also good to use as a traditional medicine in the treatment of ulcer, malaria and inflammatory condition. From that point the aim of our research was to observe cytotoxic effects of methanolic crude extracts of roots of Azadirachta indica.