The Seed of Hygrophila auriculata was collected during the month of December, 2019 from the national drug store, Tirupattur district, Tamilnadu, India.For seed extraction, hot extraction method was followed, 25g of the powdered seed materials was soaked with 50 ml in aqueous methanol (90%). The filtered extract was concentrated and stored at refrigerated condition for further studies.The presence of various phytochemicals in the Hygrophila auriculata seed extract was analyzed. The secondary metabolites of such as alkaloids, flavonoids, saponin, tannins, phenol, glycosides, terpenoids, steroids, quinone and protein were presented.The extracts of seed extract of solvent were subjected to TLC. All spots are colorless in day light but they are colored under UV light. Aqueous methanol extract showed six spots of red, yellow, pink and light yellow with RF values such as 0.3, 0.4, 0.53, 0.73.0.83 and 0.93 were respectively. Medicinal Plants produce primary and secondary metabolites withconflicting function.