In this investigation a total of 83 bacterial isolates were obtained from 80 mastitic milk samples. The identified bacteria were: 15 Staphylococcus aureus (18.3%), 7 Staphylococcus epidermidis (8.4%), 4 Staphylococcus chromogenes (4.8%), 7 Streptococcus uberis (8.4%), 9 Bacillus subtilis (10.8%), 5 Micrococcus variens (6.0%), 3 Micrococcus luteus (3.6%), 13 Escherichia coli. (15.7%), 11 Pseudomonas aerogenosa (13.3%) and 9 Klebsiella pneumoniae (10.8%). Coagulase-positive and coagulase-negative staphylococci are involved in bovine mastitis in White Nile State. Coliforms came in first place (39.9%) as a cause of bovine mastitis in White Nile State followed by Staphylococci.