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Azo dyes are the largest class of synthetic dyes and are utilized in several industries. Effluents containing dyes are released to the environment and pose harm to humans who might be exposed to these contaminants. This study aims to investigate the removal of methylene blue (MB) dye using aquatic plant Azolla pinnata. The phytoremediation of Methylene blue dye was developed using the aquatic plant Azolla pinnata as a biofilter.