Dendritic Cell Protocols

Herausgegeben:Segura, Elodie; Onai, Nobuyuki

• Gebundenes Buch

Produktbeschreibung

The third edition of thisvolume is aimed at providing both beginners and more experienced researchers achoice of methods to isolate and analyze dendritic cells(DC). An introductoryreview provides an overview of recent advances in the characterization of DCsubsets in mouse and human. While additional chapters provide methods toculture human and mouse dendritic cells, protocols for the isolation ofdendritic cells, the isolation of dendritic cell progenitors from mouse, andthe purification of dendritic cells from human blood. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.

Authoritative andcutting-edge, Dendritic CellProtocols, Third Edition aims to ensure successful results in the furtherstudy of this vital field.

Produktdetails

• Methods in Molecular Biology 1423
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-3604-5
• 3. Aufl.
• Seitenzahl: 336
• Erscheinungstermin: 4. Mai 2016
• Englisch
• Abmessung: 260mm x 183mm x 24mm
• Gewicht: 760g
• ISBN-13: 9781493936045
• ISBN-10: 1493936042
• Artikelnr.: 44264636

Inhaltsangabe

Review of Mouse and Human Dendritic Cell Subsets.- InVitro Generation of Human Xcr1⁺ Dendritic Cells from Cd34⁺Hematopoietic Progenitors.- Derivationand Utilization Of Functional Cd8⁺ Dendritic Cell Lines.- Isolationof Dendritic Cell Progenitor and Bone Marrow Progenitor Cells from Mouse.- TheIsolation and Enrichment of Large Numbers of Highly Purified Mouse SpleenDendritic Cell Populations and their InVitro Equivalents.- DendriticCell Subsets Purification from Human Tonsils and Lymph Nodes.- Isolationand Identification of Conventional Dendritic Cell Subsets from the Intestine ofMice and Men.- Isolationof Human Skin Dendritic Cell Subsets.- Isolation of Mouse Dendritic Cell Subsets andMacrophages from Skin.- Isolation of Conventional Dendritic Cells fromMouse Lungs.- Purificationof Human Dendritic Cell Subsets from Peripheral Blood.- Protocols for the Identification andIsolation ofAntigen-Presenting Cells in Human and Mouse Tissues.- Measurement of Export to the Cytosol in DendriticCells Using a Cytofluorimetry-Based Assay.- Cross-Presentation Assay for Human Dendritic Cells.- Analysis ofIntracellular Trafficking of Dendritic Cell Receptors for Antigen Targeting.- Characterizationof Dendritic Cell Subsets through Gene Expression Analysis.- In Vivo Ablation of a Dendritic Cell SubsetExpressing a Chemokine Receptor, Xcr1.- In Vivo Analysis Of IntestinalMononuclear Phagocytes.- In Vivo Imaging Of Cutaneous Dcs In Mice.- Analysis of Dendritic Cell Function by Clec9a-DtrTransgenic Mice.- Analysisof Dc Functions Using Cd205-Dtr Knock-In Mice.- Generation of Humanized Mice for Analysis ofHuman Dendritic Cells.