The prevalence of dental caries is still high in many developing countries affecting mainly the low-income population. Knowledge of dental caries prevention among dental practitioners is one of the main aspects for the effectiveness in the provision of preventive measures. This is the first study to evaluate the application of preventive measures in regards of knowledge and impediments in dental caries prevention and investigate the provision of oral health care services among Dominican dental practitioners in the Dominican Republic. The analysis is based on a cross-sectional study and data collection from self-administered surveys to Dominican dental practitioners working for the private sector.