Anchorage is one of the limiting factors in orthodontics and its control is essential for successful orthodontic treatment. Micro-implants, as an alternative method for absolute orthodontic anchorage, have been extensively used in the last few years. Despite the great interest in anchorage control with self-tapping screws, very few studies have been performed for evaluating and measuring the anatomical sites for a safe implantation of the micro-implants in the interradicular spaces of the maxillary and mandibular arches. So the present study aimed to provide an anatomical map to be used as a guideline to assist the clinician in the determination of safe locations for micro-implant placement between the roots of the posterior teeth in maxillary and mandibular arch.