Zimmer, G: Development of a CellChip based Multi-Well-Type B
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- Englisch ausgewählt
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In this work a novel multi-purpose platform for the
three-dimensional cultivation of cells is described,
its main component being a polymer chip (CellChip)
that features a microstructured area of 1-2cm2.
The microstructured area is realized as a grid of
micro-containers measuring 300µm in depth, length
and height. Depending on the cells and the chip type
used up to 7 106 cells can be cultivated on one chip.
For the facilitation of nutrient and oxygen supply
the CellChip can be inserted into a bioreactor
housing that can be tailored to the specific
application. For drug screening and other high-
throughput purposes a bioreactor with microtiter
plate-footprint was developed in this work. Up to 12
CellChips can be incorporated into this bioreactor
with independent nutrient and oxygen supply.
three-dimensional cultivation of cells is described,
its main component being a polymer chip (CellChip)
that features a microstructured area of 1-2cm2.
The microstructured area is realized as a grid of
micro-containers measuring 300µm in depth, length
and height. Depending on the cells and the chip type
used up to 7 106 cells can be cultivated on one chip.
For the facilitation of nutrient and oxygen supply
the CellChip can be inserted into a bioreactor
housing that can be tailored to the specific
application. For drug screening and other high-
throughput purposes a bioreactor with microtiter
plate-footprint was developed in this work. Up to 12
CellChips can be incorporated into this bioreactor
with independent nutrient and oxygen supply.
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