Differential Display Methods and Protocols
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Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may…mehr

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Produktbeschreibung
Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an "open"-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms-from bacteria, fungi, insects, fish, plants, to mammals-even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious "cottage-industry" academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.
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"The variety of applications...is so broad that almost every newcomer...can use these protocols as a starting point for a specific study...highly recommended for purchase by everyone interested in the comparative gene expression, and especially to newcomers to the field." -Trends in Biotechnology From Reviews of the First Edition... "...really helpful for all geneticists and cellular and molecular biologists...a precious book which should be in all hands!" -Cellular and Molecular Biology "...For any would-be gene hunter thinking of or already practicing differential display, this book will provide an invaluable and long overdue guide to the technique." -Molecular Medicine "....an ideal resource for any research group interested in using DD." -Microbiology Today "...highly recommend[ed]...for purchase by everyone interested in the comparative gene expression,, and especially to newcomers to the field." -Trends in Biotechnology